Surface-enhanced resonance Raman spectroscopy and spectroscopy study of redox-induced conformational equilibrium of cytochrome c adsorbed on DNA-modified metal electrode

	Surface-enhanced resonance Raman spectroscopy and spectroscopy study of redox-induced conformational equilibrium of cytochrome c adsorbed on DNA-modified metal electrode
	Jiang X ; Wang YL ; Qu XH ; Dong SJ
刊名	biosensors & bioelectronics
	2006
卷号	22 期号:1 页码:49-55
关键词	SELF-ASSEMBLED MONOLAYERS MODIFIED GOLD ELECTRODES DIRECT ELECTROCHEMISTRY SILVER ELECTRODES ORIENTATION CHEMISTRY PROTEINS DYNAMICS FTIR FILM
ISSN号	0956-5663
通讯作者	dong sj
中文摘要	the redox-induced conformational equilibrium of cytochrome c (cyt c) adsorbed on dna-modified metal electrode and the interaction mechanism of dna with cyt c have been studied by electrochemical, spectroscopic and spectroelectrochemical techniques. the results indicate that the external electric field induces potential-dependent coordination equilibrium of the adsorbed cyt c between its oxidized state (with native six-coordinate low-spin and non-native five-coordinate high-spin heme configuration) and its reduced state (with native six-coordinate low-spin heme configuration) on dna-modified metal electrode. the strong interactions between dna and cyt c induce the self-aggregation of cyt c adsorbed on dna. the orientational distribution of cyt c adsorbed on dna-modified metal electrode is potential-dependent, which results in the deviation from an ideal nernstian behavior of the adsorbed cyt c at high electrode potentials. the electric-field-induced increase in the activation barrier of proton-transfer steps attributed to the rearrangement of the hydrogen bond network and the self-aggregation of cyt c upon adsorption on dna-modified electrode strongly decrease the interfacial electron transfer rate.
收录类别	SCI
语种	英语
WOS记录号	WOS:000241040600007
公开日期	2010-08-17
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/16075]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Jiang X,Wang YL,Qu XH,et al. Surface-enhanced resonance Raman spectroscopy and spectroscopy study of redox-induced conformational equilibrium of cytochrome c adsorbed on DNA-modified metal electrode[J]. biosensors & bioelectronics,2006,22(1):49-55.
APA	Jiang X,Wang YL,Qu XH,&Dong SJ.(2006).Surface-enhanced resonance Raman spectroscopy and spectroscopy study of redox-induced conformational equilibrium of cytochrome c adsorbed on DNA-modified metal electrode.biosensors & bioelectronics,22(1),49-55.
MLA	Jiang X,et al."Surface-enhanced resonance Raman spectroscopy and spectroscopy study of redox-induced conformational equilibrium of cytochrome c adsorbed on DNA-modified metal electrode".biosensors & bioelectronics 22.1(2006):49-55.