Using hybrid transcription factors to study gene function in rice

doi:10.1007/s11427-015-4937-x

CORC > 中国农业科学院 > 作物科学研究所

	Using hybrid transcription factors to study gene function in rice
	Zhao Tao 1; Liu Jun 1; Li Hongyu 1; Lin Jianzhong 2; Bian Mingdi 3; Zhang Chunyu 1; Zhang Yongxing 1; Peng Yuchong 2; Liu Bin 1; Lin Chentao 4
刊名	Science China. Life Sciences
	2015
卷号	58 期号:11 页码:1160-1162
关键词	sequences proteins transgenic
ISSN号	1674-7305
DOI	10.1007/s11427-015-4937-x
英文摘要	Transcription factors (TF) play important roles in plant growth and development, which determine crop productivity, quality, and stress tolerance. However, the basic mechanism governing transcription regulation by TF remains largely unclear in plants. It is well known that TF proteins contain at least two functional domains, the DNA-binding domain and the transcription regulatory domain. The VP16 transcription activation domain of human Herpes simplex virus and the EAR transcription suppression domain of many plant TF proteins are two of the best-known transcription regulatory domains. It has been reported that both VP16 and EAR domains exert their respective transcription activation/repression functions in heterologous protein and/or cellular environment, supporting a hypothesis that the transcription regulatory domains function independent of other functional domains of TF proteins. However, this hypothesis has not been examined systematically. We reasoned that a phenotypic analysis of transgenic expression of all or most TF genes from a plant species in the form of fusion proteins, which are composed of the coding sequences of TFs fused to the VP16 or EAR domain, would allow a systematic test of this hypothesis, and we reported here the overall result of such an experiment.
学科主题	细胞生物学 ; 生物化学
语种	中文
内容类型	期刊论文
源URL	[http://ir.nais.net.cn/handle/2HMLN22E/131415]
专题	作物科学研究所
作者单位	1.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081; 2.College of Biology,Hunan University, Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, Changsha, 410082; 3.Laboratory of Soil and Plant Molecular Genetics, College of Plant Science, Jilin University, Changchun, 130062; 4.Department of Molecular, Cell & Developmental Biology, University of California, USA, Los Angeles, CA90095
推荐引用方式 GB/T 7714	Zhao Tao,Liu Jun,Li Hongyu,et al. Using hybrid transcription factors to study gene function in rice[J]. Science China. Life Sciences,2015,58(11):1160-1162.
APA	Zhao Tao.,Liu Jun.,Li Hongyu.,Lin Jianzhong.,Bian Mingdi.,...&Lin Chentao.(2015).Using hybrid transcription factors to study gene function in rice.Science China. Life Sciences,58(11),1160-1162.
MLA	Zhao Tao,et al."Using hybrid transcription factors to study gene function in rice".Science China. Life Sciences 58.11(2015):1160-1162.