A genetic evidence of chromosomal fragment from bridge parent existing in substitution lines between two common wheat varieties

doi:10.1016/S2095-3119(15)61038-3

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	A genetic evidence of chromosomal fragment from bridge parent existing in substitution lines between two common wheat varieties
	Zhao Pei 1; Wang Ke 1; Lin Zhishan 1; Liu Huiyun 1; Li Xin 1; Du Lipu 1; Yan Yueming 2; Ye Xingguo 1
刊名	Journal of Integrative Agriculture
	2016
卷号	15 期号:1 页码:10-17
关键词	wheat intracultivaral chromosome substitution lines agronomic traits high molecular weight glutenin subunits (HMW-GS) molecular markers
ISSN号	2095-3119
DOI	10.1016/S2095-3119(15)61038-3
英文摘要	Locating of important agronomic genes onto chromosome is helpful for efficient development of new wheat varieties. Wheat chromosome substitution lines between two varieties have been widely used for locating genes because of their distinctive advantages in genetic analysis, compared with the aneuploid genetic materials. Apart from the substituted chromosome, the other chromosomes between the substitution lines and their recipient parent should be identical, which eases the gene locating practice. In this study, a set of chromosome substitution lines with cv. Wichita (WI) as the recipient parent and cv. Cheyenne (CNN) as the donor parent were studied for the composition of high molecular weight glutenin subunits (HMW-GS) as well as a range of agronomic important traits. Results revealed that the substitution lines of WI(CNN5D), WI(CNN6A) and WI(CNN7B) had higher plant heights than the two parents of WI and CNN, and WI(CNN3D) had later maturity than the parents. By sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis, a substitution line WI(CNN5B) was found to contain different HMW-GS patterns from its two parents, in which 1By9 was replaced by 1By8 on chromosome 1BL. Simple sequence repeat (SSR) analysis confirmed that the variation on 1BL in WI(CNN5B) was originated from Chinese Spring (CS). It is concluded that chromosomal fragments from bridge material and donor parent were quite often retained in intracultivaral chromosome substitution lines except the substituting chromosomes.
语种	中文
内容类型	期刊论文
源URL	[http://ir.nais.net.cn/handle/2HMLN22E/131143]
专题	作物科学研究所
作者单位	1.Institute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facility of Crop Gene Resources and Genetic Improvement, Beijing, 100081; 2.Key Laboratory of Genetics and Biotechnology, College of Life Science, Capital Normal University, Beijing, 100048
推荐引用方式 GB/T 7714	Zhao Pei,Wang Ke,Lin Zhishan,et al. A genetic evidence of chromosomal fragment from bridge parent existing in substitution lines between two common wheat varieties[J]. Journal of Integrative Agriculture,2016,15(1):10-17.
APA	Zhao Pei.,Wang Ke.,Lin Zhishan.,Liu Huiyun.,Li Xin.,...&Ye Xingguo.(2016).A genetic evidence of chromosomal fragment from bridge parent existing in substitution lines between two common wheat varieties.Journal of Integrative Agriculture,15(1),10-17.
MLA	Zhao Pei,et al."A genetic evidence of chromosomal fragment from bridge parent existing in substitution lines between two common wheat varieties".Journal of Integrative Agriculture 15.1(2016):10-17.