FUNCTIONAL CHARACTERIZATION AND IMMUNOLOCALIZATION OF ODORANT BINDING PROTEIN 1 IN THE LUCERNE PLANT BUG, Adelphocoris lineolatus (GOEZE)

doi:10.1002/arch.20427

CORC > 中国农业科学院 > 植物保护研究所

	FUNCTIONAL CHARACTERIZATION AND IMMUNOLOCALIZATION OF ODORANT BINDING PROTEIN 1 IN THE LUCERNE PLANT BUG, Adelphocoris lineolatus (GOEZE)
	Gu, Shao-Hua 1; Wang, Wei-Xuan 2; Wang, Gui-Rong 1; Zhang, Xue-Ying 1; Guo, Yu-Yuan 1; Zhang, Ziding 2; Zhou, Jing-Jiang 3; Zhang, Yong-Jun 1
刊名	ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
	2011
卷号	77 期号:2 页码:81-98
关键词	Adelphocoris lineolatus (Goeze) odorant binding protein protein expression expression pattern fluorescence binding molecular model Immunocytochemistry
ISSN号	0739-4462
DOI	10.1002/arch.20427
通讯作者	Gu, Shao-Hua
英文摘要	In the insect phylum, the relationships between individuals and their environment are often modulated by chemical communication. Odorant binding proteins (OBPs) are widely and robustly expressed in insect olfactory organs and play a key role in chemosensing and transporting hydrophobic odorants across the sensillum lymph to the olfactory receptor neuron. In this study, a novel OBP gene (AlinOBP1) in the lucerne plant bug, Adelphocoris lineolatus was identified, cloned and expressed. Real-time PCR results indicated that the expression level of AlinOBP1 gene differed in each developmental stage (from first instar to adult) and was predominantly expressed in the antennae of adults. The expression level of AlinOBP1 was 1.91 times higher in male antennae than in female antennae. The binding properties of AlinOBP1 with 114 odorants were measured using a fluorescence probe, N-phenyl-1-naphthylamine (1-NPN), with fluorescence competitive binding. The results revealed that AlinOBP1 exhibits high binding abilities with two major putative pheromone components, ethyl butyrate and trans-2-hexenyl butyrate. In addition, it was observed that six volatiles released from cotton, octanal, nonanal, decanal, 2-ethyl-1-hexanol, beta-caryophyllene and beta-ionone also bind to AlinOBP1. Immunocytochemistry analysis showed that AlinOBP1 was expressed in the sensillum lymph of sensilla trichodica and sensilla basiconca. Our results demonstrate that AlinOBP1 may function as a carrier in the chemoperception of the lucerne plant bug. (C) 2011 Wiley Periodicals, Inc.
学科主题	Biochemistry & Molecular Biology ; Entomology ; Physiology ; BIOCHEMISTRY & MOLECULAR BIOLOGY ; ENTOMOLOGY ; PHYSIOLOGY
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000290632500004
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/12358]
专题	植物保护研究所
作者单位	1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China 2.China Agr Univ, Coll Biol Sci, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China 3.Rothamsted Res, Dept Biol Chem, Harpenden AL5 2JQ, Herts, England
推荐引用方式 GB/T 7714	Gu, Shao-Hua,Wang, Wei-Xuan,Wang, Gui-Rong,et al. FUNCTIONAL CHARACTERIZATION AND IMMUNOLOCALIZATION OF ODORANT BINDING PROTEIN 1 IN THE LUCERNE PLANT BUG, Adelphocoris lineolatus (GOEZE)[J]. ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY,2011,77(2):81-98.
APA	Gu, Shao-Hua.,Wang, Wei-Xuan.,Wang, Gui-Rong.,Zhang, Xue-Ying.,Guo, Yu-Yuan.,...&Zhang, Yong-Jun.(2011).FUNCTIONAL CHARACTERIZATION AND IMMUNOLOCALIZATION OF ODORANT BINDING PROTEIN 1 IN THE LUCERNE PLANT BUG, Adelphocoris lineolatus (GOEZE).ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY,77(2),81-98.
MLA	Gu, Shao-Hua,et al."FUNCTIONAL CHARACTERIZATION AND IMMUNOLOCALIZATION OF ODORANT BINDING PROTEIN 1 IN THE LUCERNE PLANT BUG, Adelphocoris lineolatus (GOEZE)".ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY 77.2(2011):81-98.