The SINA E3 Ligase OsDIS1 Negatively Regulates Drought Response in Rice

doi:10.1104/pp.111.180893

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	The SINA E3 Ligase OsDIS1 Negatively Regulates Drought Response in Rice
	Ning, Yuese 1,2,3,4; Jantasuriyarat, Chachawan 4; Zhao, Qingzhen ; Zhang, Huawei ; Chen, Songbiao 4; Liu, Jinling 1,2; Liu, Lijing ; Tang, Sanyuan ; Park, Chan Ho 4; Wang, Xuejun 1,2
刊名	PLANT PHYSIOLOGY
	2011
卷号	157 期号:1 页码:242-255
ISSN号	0032-0889
DOI	10.1104/pp.111.180893
通讯作者	Ning, Yuese
英文摘要	Ubiquitin-regulated protein degradation is a critical regulatory mechanism that controls a wide range of biological processes in plants. Here, we report that OsDIS1 (for Oryza sativa drought-induced SINA protein 1), a C3HC4 RING finger E3 ligase, is involved in drought-stress signal transduction in rice (O. sativa). The expression of OsDIS1 was up-regulated by drought treatment. In vitro ubiquitination assays showed that OsDIS1 possessed E3 ubiquitin ligase activity and that the conserved region of the RING finger was required for the activity. Transient expression assays in Nicotiana benthamiana leaves and rice protoplasts indicated that OsDIS1 was localized predominantly in the nucleus. Overexpression of OsDIS1 reduced drought tolerance in transgenic rice plants, while RNA interference silencing of OsDIS1 enhanced drought tolerance. Microarray analysis revealed that a large number of drought-responsive genes were induced or suppressed in the OsDIS1 overexpression plants under normal and drought conditions. Yeast two-hybrid screening showed that OsDIS1 interacted with OsNek6 (for O. sativa NIMA-related kinase 6), a tubulin complex-related serine/threonine protein kinase. Coexpression assays in N. benthamiana leaves indicated that OsNek6 was degraded by OsDIS1 via the 26S proteasome-dependent pathway and that this degradation was abolished by the OsDIS1(H71Y) mutation, which is essential for its E3 ligase activity. Together, these results demonstrate that OsDIS1 plays a negative role in drought stress tolerance through transcriptional regulation of diverse stress-related genes and possibly through posttranslational regulation of OsNek6 in rice.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	AMER SOC PLANT BIOLOGISTS
WOS记录号	WOS:000294491800019
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/12341]
专题	植物保护研究所
作者单位	1.Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China 2.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res, State Key Lab Plant Genom, Beijing 100101, Peoples R China 3.Chinese Acad Agr Sci, Inst Plant Protect, State Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China 4.Ohio State Univ, Dept Plant Pathol, Columbus, OH 43210 USA
推荐引用方式 GB/T 7714	Ning, Yuese,Jantasuriyarat, Chachawan,Zhao, Qingzhen,et al. The SINA E3 Ligase OsDIS1 Negatively Regulates Drought Response in Rice[J]. PLANT PHYSIOLOGY,2011,157(1):242-255.
APA	Ning, Yuese.,Jantasuriyarat, Chachawan.,Zhao, Qingzhen.,Zhang, Huawei.,Chen, Songbiao.,...&Wang, Guo-Liang.(2011).The SINA E3 Ligase OsDIS1 Negatively Regulates Drought Response in Rice.PLANT PHYSIOLOGY,157(1),242-255.
MLA	Ning, Yuese,et al."The SINA E3 Ligase OsDIS1 Negatively Regulates Drought Response in Rice".PLANT PHYSIOLOGY 157.1(2011):242-255.