The U-Box/ARM E3 Ligase PUB13 Regulates Cell Death, Defense, and Flowering Time in Arabidopsis

doi:10.1104/pp.111.192617

CORC > 中国农业科学院 > 植物保护研究所

	The U-Box/ARM E3 Ligase PUB13 Regulates Cell Death, Defense, and Flowering Time in Arabidopsis
	Li, Wei 1,2,4,5; Ahn, Il-Pyung 4; Ning, Yuese 1,2,3; Park, Chan-Ho 4; Zeng, Lirong 1,4,6; Whitehill, Justin G. A.4; Lu, Haibin 5; Zhao, Qingzhen 7; Ding, Bo 4; Xie, Qi 7
刊名	PLANT PHYSIOLOGY
	2012
卷号	159 期号:1 页码:239-250
ISSN号	0032-0889
DOI	10.1104/pp.111.192617
通讯作者	Li, Wei
英文摘要	The components in plant signal transduction pathways are intertwined and affect each other to coordinate plant growth, development, and defenses to stresses. The role of ubiquitination in connecting these pathways, particularly plant innate immunity and flowering, is largely unknown. Here, we report the dual roles for the Arabidopsis (Arabidopsis thaliana) Plant U-box protein13 (PUB13) in defense and flowering time control. In vitro ubiquitination assays indicated that PUB13 is an active E3 ubiquitin ligase and that the intact U-box domain is required for the E3 ligase activity. Disruption of the PUB13 gene by T-DNA insertion results in spontaneous cell death, the accumulation of hydrogen peroxide and salicylic acid (SA), and elevated resistance to biotrophic pathogens but increased susceptibility to necrotrophic pathogens. The cell death, hydrogen peroxide accumulation, and resistance to necrotrophic pathogens in pub13 are enhanced when plants are pretreated with high humidity. Importantly, pub13 also shows early flowering under middle-and long-day conditions, in which the expression of SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 and FLOWERING LOCUS T is induced while FLOWERING LOCUS C expression is suppressed. Finally, we found that two components involved in the SA-mediated signaling pathway, SID2 and PAD4, are required for the defense and flowering-time phenotypes caused by the loss of function of PUB13. Taken together, our data demonstrate that PUB13 acts as an important node connecting SA-dependent defense signaling and flowering time regulation in Arabidopsis.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	AMER SOC PLANT BIOLOGISTS
WOS记录号	WOS:000303657100021
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/12268]
专题	植物保护研究所
作者单位	1.Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China 2.Hunan Agr Univ, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Hunan, Peoples R China 3.Chinese Acad Agr Sci, Inst Plant Protect, State Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China 4.Ohio State Univ, Dept Plant Pathol, Columbus, OH 43210 USA 5.Natl Inst Biol Sci, Beijing 102206, Peoples R China 6.Univ Arkansas, Dept Biol, Little Rock, AR 72204 USA 7.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res, State Key Lab Plant Genom, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Li, Wei,Ahn, Il-Pyung,Ning, Yuese,et al. The U-Box/ARM E3 Ligase PUB13 Regulates Cell Death, Defense, and Flowering Time in Arabidopsis[J]. PLANT PHYSIOLOGY,2012,159(1):239-250.
APA	Li, Wei.,Ahn, Il-Pyung.,Ning, Yuese.,Park, Chan-Ho.,Zeng, Lirong.,...&Wang, Guo-Liang.(2012).The U-Box/ARM E3 Ligase PUB13 Regulates Cell Death, Defense, and Flowering Time in Arabidopsis.PLANT PHYSIOLOGY,159(1),239-250.
MLA	Li, Wei,et al."The U-Box/ARM E3 Ligase PUB13 Regulates Cell Death, Defense, and Flowering Time in Arabidopsis".PLANT PHYSIOLOGY 159.1(2012):239-250.