Molecular identification and differential expression of sensory neuron membrane proteins in the antennae of the black cutworm moth Agrotis ipsilon

doi:10.1016/j.jinsphys.2013.02.003

CORC > 中国农业科学院 > 植物保护研究所

	Molecular identification and differential expression of sensory neuron membrane proteins in the antennae of the black cutworm moth Agrotis ipsilon
	Gu, Shao-Hua 1; Yang, Ruo-Nan 1; Guo, Meng-Bo 1; Wang, Gui-Rong 1; Wu, Kong-Ming 1; Guo, Yu-Yuan 1; Zhou, Jing-Jiang 2; Zhang, Yong-Jun 1
刊名	JOURNAL OF INSECT PHYSIOLOGY
	2013
卷号	59 期号:4 页码:430-443
关键词	Agrotis ipsilon Sensory neuron membrane protein Molecular identification Quantitative real-time PCR Immunocytochemistry
ISSN号	0022-1910
DOI	10.1016/j.jinsphys.2013.02.003
通讯作者	Gu, Shao-Hua
英文摘要	The insect sensory neuron membrane proteins (SNMPs) SNMP1 and SNMP2 are transmembrane domain-containing proteins and are homologs of the vertebrate CD36 transmembrane proteins. It has been suggested that SNMPs play a significant role in insect chemoreception. Previous studies have demonstrated that SNMP1 is expressed in the pheromone-sensitive olfactory receptor neurons (ORNs), whereas SNMP2 is expressed in the supporting cells. In this study, we identified two full-length SNMP transcripts, AipsSNMP1 and AipsSNMP2, in the black cutworm moth Agrotis ipsilon (Hufnagel). The qRT-PCR results indicated that the AipsSNMP1 and AipsSNMP2 transcripts were expressed significantly higher in the antennae than in other tissues of both sexes. The expression of AipsSNMP1 and AipsSNMP2 in the antennae from different development stages of both sexes was investigated and was shown to begin to express in the pupae stage from 3 days before emergence and then increased dramatically at the day of the emergence, and the high expression levels were maintained during the following 4 days after the emergence in both sexes. The mating status had no effect on the expression levels of the AipsSNMP1 and AipsSNMP2 transcripts. Consistent with previous in situ hybridization studies in other Lepidoptera insects, our immunolocalization results at protein level demonstrated that both AipsSNMP1 and AipsSNMP2 were expressed in pheromone-sensitive sensilla trichodea but with a completely different expression profile. AipsSNMP1 is more uniformed and highly expressed along the membrane of the ORN dendrites, whereas AipsSNMP2 is widely distributed at the bottom of the sensilla trichodea and highly localized in the sensillum lymph. Our studies provide further detailed evidence for the involvement and general functional role of insect SNMPs in the detection of sex pheromones and general odorant molecules. (c) 2013 Elsevier Ltd. All rights reserved.
学科主题	Entomology ; Physiology ; Zoology ; ENTOMOLOGY ; PHYSIOLOGY
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000317797100007
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/12154]
专题	植物保护研究所
作者单位	1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China 2.Rothamsted Res, Dept Biol Chem & Crop Protect, Harpenden AL5 2JQ, Herts, England
推荐引用方式 GB/T 7714	Gu, Shao-Hua,Yang, Ruo-Nan,Guo, Meng-Bo,et al. Molecular identification and differential expression of sensory neuron membrane proteins in the antennae of the black cutworm moth Agrotis ipsilon[J]. JOURNAL OF INSECT PHYSIOLOGY,2013,59(4):430-443.
APA	Gu, Shao-Hua.,Yang, Ruo-Nan.,Guo, Meng-Bo.,Wang, Gui-Rong.,Wu, Kong-Ming.,...&Zhang, Yong-Jun.(2013).Molecular identification and differential expression of sensory neuron membrane proteins in the antennae of the black cutworm moth Agrotis ipsilon.JOURNAL OF INSECT PHYSIOLOGY,59(4),430-443.
MLA	Gu, Shao-Hua,et al."Molecular identification and differential expression of sensory neuron membrane proteins in the antennae of the black cutworm moth Agrotis ipsilon".JOURNAL OF INSECT PHYSIOLOGY 59.4(2013):430-443.