The Preferential Binding of a Sensory Organ Specific Odorant Binding Protein of the Alfalfa Plant Bug Adelphocoris lineolatus AlinOBP10 to Biologically Active Host Plant Volatiles

doi:10.1007/s10886-013-0333-9

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	The Preferential Binding of a Sensory Organ Specific Odorant Binding Protein of the Alfalfa Plant Bug Adelphocoris lineolatus AlinOBP10 to Biologically Active Host Plant Volatiles
	Sun, Liang 1,2; Gu, Shao-Hua 2; Xiao, Hai-Jun 2; Zhou, Jing-Jiang 3; Guo, Yu-Yuan 2; Liu, Ze-Wen 1; Zhang, Yong-Jun 2
刊名	JOURNAL OF CHEMICAL ECOLOGY
	2013
卷号	39 期号:9 页码:1221-1231
关键词	Adelphocoris lineolatus Odorant-binding proteins Fluorescence competitive binding assay EAG recording Y-tube olfactometer
ISSN号	0098-0331
DOI	10.1007/s10886-013-0333-9
通讯作者	Sun, Liang
英文摘要	Semiochemicals such as sex pheromones and plant volatiles are crucial components of insect mating systems and host plant localization. In the olfactory signal transduction pathway, odorant-binding proteins (OBPs) are important elements that function in the first step of the pathway by carrying hydrophobic semiochemicals across the sensillum lymph to the olfactory receptors (ORs). In this study, we examined the binding affinities of semiochemicals to AlinOBP10, a putative OBP from the alfalfa plant bug, Adelphocoris lineolatus, that we demonstrate is expressed mainly in sensory organs. We then characterized the biological activities of the high affinity semiochemicals by measuring their electrophysiological activities in antennae and behavioral responses in the plant bug. AlinOBP10 displayed weak binding affinities to two major putative pheromone components, hexyl butyrate and (E)-2-hexenyl butyrate. In contrast, AlinOBP10 exhibited higher binding affinities to six host plant volatiles, namely myrcene, beta-pinene, beta-ionone, 3-hexanone, (E)-2-hexenal, and 1-hexanol. The biological activities of these six putative ligands were further studied in electroantennogram recordings and Y-tube olfactometer trials. The three compounds, (E)-2-hexenal, 1-hexanol, and 3-hexanone elicited strong electrophysiological responses, but elicited distinct behaviors. While 3-hexanone was attractive to female adults, (E)-2-hexenal and 1-hexanol were significant repellents. Although a weak electrophysiological response was elicited with beta-pinene, it was a strong repellent. These results demonstrate that AlinOBP10 can interact with attractants, as well as repellents, with some specificity toward plant volatiles over sex pheromones.
学科主题	Biochemistry & Molecular Biology ; Ecology ; BIOCHEMISTRY & MOLECULAR BIOLOGY ; ECOLOGY
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000325247400010
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/12100]
专题	植物保护研究所
作者单位	1.Nanjing Agr Univ, Coll Plant Protect, Minist Educ, Key Lab Integrated Management Crop Dis & Pests, Nanjing 210095, Jiangsu, Peoples R China 2.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China 3.Rothamsted Res, Dept Biol Chem & Crop Protect, Harpenden AL5 2JQ, Herts, England
推荐引用方式 GB/T 7714	Sun, Liang,Gu, Shao-Hua,Xiao, Hai-Jun,et al. The Preferential Binding of a Sensory Organ Specific Odorant Binding Protein of the Alfalfa Plant Bug Adelphocoris lineolatus AlinOBP10 to Biologically Active Host Plant Volatiles[J]. JOURNAL OF CHEMICAL ECOLOGY,2013,39(9):1221-1231.
APA	Sun, Liang.,Gu, Shao-Hua.,Xiao, Hai-Jun.,Zhou, Jing-Jiang.,Guo, Yu-Yuan.,...&Zhang, Yong-Jun.(2013).The Preferential Binding of a Sensory Organ Specific Odorant Binding Protein of the Alfalfa Plant Bug Adelphocoris lineolatus AlinOBP10 to Biologically Active Host Plant Volatiles.JOURNAL OF CHEMICAL ECOLOGY,39(9),1221-1231.
MLA	Sun, Liang,et al."The Preferential Binding of a Sensory Organ Specific Odorant Binding Protein of the Alfalfa Plant Bug Adelphocoris lineolatus AlinOBP10 to Biologically Active Host Plant Volatiles".JOURNAL OF CHEMICAL ECOLOGY 39.9(2013):1221-1231.