Population Genetic Analysis of Blumeria graminis f. sp tritici in Qinghai Province, China

doi:10.1016/S2095-3119(13)60591-2

CORC > 中国农业科学院 > 植物保护研究所

	Population Genetic Analysis of Blumeria graminis f. sp tritici in Qinghai Province, China
	Xu Zhi 1; Duan Xia-yu 1; Zhou Yi-lin 1; Guo Qing-yun 2; Yao Qiang 2; Cao Shi-qin 3
刊名	JOURNAL OF INTEGRATIVE AGRICULTURE
	2014
卷号	13 期号:9 页码:1952-1961
关键词	Blumeria graminis f. sp tritici virulence single nucleotide polymorphisms haplotype
ISSN号	2095-3119
DOI	10.1016/S2095-3119(13)60591-2
通讯作者	Xu Zhi
英文摘要	To gain more precise information about molecular genetic variation for wild populations of Blumeria graminis f. sp. tritici from Qinghai Province, China, 38 single-colony isolates were purified from samples collected from Haidong District, Xining City and Hainan Tibetan Autonomous Prefecture in 2010. The virulence of 21 isolates among them was tested at seedling stage on 34 wheat cultivars (lines) carrying known powdery mildew (Pm) resistant genes. The results showed that V1a,V3a,V3c,V3e,V5a, V6, V7, V8 and V19 had high virulence frequencies (>75%), indicating a wide distribution; and V1c, V5b, V12, V13, V16, V21, VXBD, V2+6, V2+Mld and V4+8, with less distribution, appeared to be lower in frequencies (0-20%). The Nei's gene diversity (H), Shannon's information index (1) and the percentage of polymorphic loci (P) were 0.23, 0.35 and 67.65%, respectively, which revealed a virulent diversity. The results from single nucleotide polymorphisms (SNPs) of 38 isolates showed that three housekeeping genes were found to contain a total of 9 SNP sites. 10 haplotypes (H1-H10) were inferred from the concatenated sequences, with 1 haplotype (H1) comprising of over 55% of Qinghai population. Phylogenic analysis did not show obvious geographical subdivision between the isolates. A multilocus haplotype network presented a radial structure, with H1 in the central as an inferred ancestor. Using analysis of molecular variance (AMOVA), we found 1.63% of the total variation was among populations and 98.37% within populations, with a low fixations index (F-ST=0.01634, P<0.05). This revealed a relatively high genetic diversity but a low genetic divergence in Qinghai population. Moreover, the molecular data on gene flow (Nm=6.32) confirmed the migration of pathogen populations among areas in Qinghai Province.
学科主题	Agriculture, Multidisciplinary
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000341740900013
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/12047]
专题	植物保护研究所
作者单位	1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China 2.Qinghai Acad Agr & Forestry, Inst Plant Protect, Xining 810016, Peoples R China 3.Gansu Acad Agr Sci, Inst Plant Protect, Lanzhou 730070, Peoples R China
推荐引用方式 GB/T 7714	Xu Zhi,Duan Xia-yu,Zhou Yi-lin,et al. Population Genetic Analysis of Blumeria graminis f. sp tritici in Qinghai Province, China[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2014,13(9):1952-1961.
APA	Xu Zhi,Duan Xia-yu,Zhou Yi-lin,Guo Qing-yun,Yao Qiang,&Cao Shi-qin.(2014).Population Genetic Analysis of Blumeria graminis f. sp tritici in Qinghai Province, China.JOURNAL OF INTEGRATIVE AGRICULTURE,13(9),1952-1961.
MLA	Xu Zhi,et al."Population Genetic Analysis of Blumeria graminis f. sp tritici in Qinghai Province, China".JOURNAL OF INTEGRATIVE AGRICULTURE 13.9(2014):1952-1961.