Identification and Characterization of Suppressor Mutants of spl11-Mediated Cell Death in Rice

doi:10.1094/MPMI-08-13-0259-R

CORC > 中国农业科学院 > 植物保护研究所

	Identification and Characterization of Suppressor Mutants of spl11-Mediated Cell Death in Rice
	Shirsekar, Gautam S.1; Vega-Sanchez, Miguel E.1; Bordeos, Alicia 2; Baraoidan, Marietta 2; Swisshelm, Andrew 1; Fan, Jiangbo 1,3; Park, Chan Ho 1; Leung, Hei 2; Wang, Guo-Liang 1,3
刊名	MOLECULAR PLANT-MICROBE INTERACTIONS
	2014
卷号	27 期号:6 页码:528-536
ISSN号	0894-0282
DOI	10.1094/MPMI-08-13-0259-R
通讯作者	Shirsekar, Gautam S.
英文摘要	Lesion mimic mutants have been used to dissect programmed cell death (PCD) and defense-related pathways in plants. The rice lesion-mimic mutant spl11 exhibits race nonspecific resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae and the fungal pathogen Magnaporthe myzae. Spl11 encodes an E3 ubiquitin ligase and is a negative regulator of PCD in rice. To study the regulation of Spl11-mediated PCD, we performed a genetic screen and identified three spl11 cell-death suppressor (sds) mutants. These suppressors were characterized for their resistance to X. oryzae pv. myzae and M. myzae and for their expression of defense-related genes. The suppression of the cell-death phenotypes was generally correlated with reduced expression of defense-related genes. When rice was challenged with avirulent isolates of M. myzae, the disease phenotype was unaffected in the sds mutants, indicating that the suppression might be Spl11-mediated pathway specific and may only be involved in basal defense. In addition, we mapped one of the suppressor mutations to a 140-kb interval on the long arm of rice chromosome 1. Identification and characterization of these sds mutants should facilitate our efforts to elucidate the Spl11-mediated PCD pathway.
学科主题	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Plant Sciences ; BIOCHEMISTRY & MOLECULAR BIOLOGY ; BIOTECHNOLOGY & APPLIED MICROBIOLOGY ; PLANT SCIENCES
语种	英语
出版者	AMER PHYTOPATHOLOGICAL SOC
WOS记录号	WOS:000335635500003
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11951]
专题	植物保护研究所
作者单位	1.Ohio State Univ, Dept Plant Pathol, Plant Mol Biol & Biotechnol Program, Columbus, OH 43210 USA 2.IRRI, Manila, Philippines 3.Chinese Acad Agr Sci, Inst Plant Protect, State Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Shirsekar, Gautam S.,Vega-Sanchez, Miguel E.,Bordeos, Alicia,et al. Identification and Characterization of Suppressor Mutants of spl11-Mediated Cell Death in Rice[J]. MOLECULAR PLANT-MICROBE INTERACTIONS,2014,27(6):528-536.
APA	Shirsekar, Gautam S..,Vega-Sanchez, Miguel E..,Bordeos, Alicia.,Baraoidan, Marietta.,Swisshelm, Andrew.,...&Wang, Guo-Liang.(2014).Identification and Characterization of Suppressor Mutants of spl11-Mediated Cell Death in Rice.MOLECULAR PLANT-MICROBE INTERACTIONS,27(6),528-536.
MLA	Shirsekar, Gautam S.,et al."Identification and Characterization of Suppressor Mutants of spl11-Mediated Cell Death in Rice".MOLECULAR PLANT-MICROBE INTERACTIONS 27.6(2014):528-536.