The Y123H substitution perturbs FvCYP51B function and confers prochloraz resistance in laboratory mutants of Fusarium verticillioides

doi:10.1111/ppa.12168

CORC > 中国农业科学院 > 植物保护研究所

	The Y123H substitution perturbs FvCYP51B function and confers prochloraz resistance in laboratory mutants of Fusarium verticillioides
	Fan, J.1,2; Chen, F.1,3; Diao, Y.1; Cools, H. J.4; Kelly, S. L.5,6; Liu, X.1
刊名	PLANT PATHOLOGY
	2014
卷号	63 期号:4 页码:952-960
关键词	azole fungicide CYP51 resistance mechanism site mutation yeast expression
ISSN号	0032-0862
DOI	10.1111/ppa.12168
通讯作者	Fan, J.
英文摘要	Fusarium verticillioides reduces corn yield and contaminates infected kernels with the toxin fumonisin, which is harmful to humans and animals. Previous research has demonstrated that F. verticillioides can be controlled by the azole fungicide prochloraz. Currently, prochloraz is used as a foliar spray to control maize disease in China, which will increase the risk of resistance. Although F. verticillioides resistance to prochloraz has not been reported in the field, possible resistance risk and mechanisms resulting in prochloraz resistance were explored in the laboratory. Four prochloraz-resistant strains of F. verticillioides were generated by successive selection on fungicide-amended media. The mycelial growth rates of the mutants were inversely related to the level of resistance. All four mutants were cross-resistant to the triazole fungicides triadimefon, tebuconazole and difenoconazole, but not to the multisite fungicide chlorothalonil or to the MAP/histidine-kinase inhibitor fungicide fludioxonil. Based on the Y123H mutation in FvCYP51B, the four resistant mutants were subdivided into two genotypes: PCZ-R1 mutants with wildtype FvCYP51B and PCZ-R2 mutants with substitution Y123H in FvCYP51B. Wildtype FvCYP51B complemented the function of native ScCYP51 in Saccharomyces cerevisiae YUG37::erg11, whereas Y123H-mutated FvCYP51B did not. For the PCZ-R1 mutants, induced expression of FvCYP51A increased resistance to prochloraz. For the PCZ-R2 mutants, disruption of FvCYP51B function by the Y123H substitution caused constitutive up-regulation of FvCYP51A expression and thus resistance to prochloraz.
学科主题	Agronomy ; Plant Sciences ; AGRONOMY ; PLANT SCIENCES
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000340660800023
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11911]
专题	植物保护研究所
作者单位	1.China Agr Univ, Dept Plant Pathol, Beijing 100193, Peoples R China 2.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China 3.Fujian Agr & Forestry Univ, Inst Plant Virol, Key Lab Plant Virol Fujian Prov, Fuzhou 350002, Peoples R China 4.Rothamsted Res, Dept Biol Chem & Crop Protect, Harpenden AL5 2JQ, Herts, England 5.Swansea Univ, Inst Life Sci, Swansea SA2 8PP, W Glam, Wales 6.Swansea Univ, Coll Med, Swansea SA2 8PP, W Glam, Wales
推荐引用方式 GB/T 7714	Fan, J.,Chen, F.,Diao, Y.,et al. The Y123H substitution perturbs FvCYP51B function and confers prochloraz resistance in laboratory mutants of Fusarium verticillioides[J]. PLANT PATHOLOGY,2014,63(4):952-960.
APA	Fan, J.,Chen, F.,Diao, Y.,Cools, H. J.,Kelly, S. L.,&Liu, X..(2014).The Y123H substitution perturbs FvCYP51B function and confers prochloraz resistance in laboratory mutants of Fusarium verticillioides.PLANT PATHOLOGY,63(4),952-960.
MLA	Fan, J.,et al."The Y123H substitution perturbs FvCYP51B function and confers prochloraz resistance in laboratory mutants of Fusarium verticillioides".PLANT PATHOLOGY 63.4(2014):952-960.