Influence of transgenic rice expressing a fused Cry1Ab/1Ac protein on frogs in paddy fields

doi:10.1007/s10646-014-1301-z

CORC > 中国农业科学院 > 植物保护研究所

	Influence of transgenic rice expressing a fused Cry1Ab/1Ac protein on frogs in paddy fields
	Wang, Jia-Mei 1,2; Chen, Xiu-Ping 1; Liang, Yu-Yong 3; Zhu, Hao-Jun 1,2; Ding, Jia-Tong 2; Peng, Yu-Fa 1
刊名	ECOTOXICOLOGY
	2014
卷号	23 期号:9 页码:1619-1628
关键词	Transgenic rice Bt protein Frog Safety assessment Non-target effect
ISSN号	0963-9292
DOI	10.1007/s10646-014-1301-z
通讯作者	Wang, Jia-Mei
英文摘要	As genetic engineering in plants is increasingly used to control agricultural pests, it is important to determine whether such transgenic plants adversely affect non-target organisms within and around cultivated fields. The cry1Ab/1Ac fusion gene from Bacillus thuringiensis (Bt) has insecticidal activity and has been introduced into rice line Minghui 63 (MH63). We evaluated the effect of transgenic cry1Ab/1Ac rice (Huahui 1, HH1) on paddy frogs by comparing HH1 and MH63 rice paddies with and without pesticide treatment. The density of tadpoles in rice fields was surveyed at regular intervals, and Cry1Ab/1Ac protein levels were determined in tissues of tadpoles and froglets collected from the paddy fields. In addition, Rana nigromaculata froglets were raised in purse nets placed within these experimental plots. The survival, body weight, feeding habits, and histological characteristics of the digestive tract of these froglets were analyzed. We found that the tadpole density was significantly decreased immediately after pesticide application, and the weight of R. nigromaculata froglets of pesticide groups was significantly reduced compared with no pesticide treatment, but we found no differences between Bt and non-Bt rice groups. Moreover, no Cry1Ab/1Ac protein was detected in tissue samples collected from 192 tadpoles and froglets representing all four experimental groups. In addition, R. nigromaculata froglets raised in purse seines fed primarily on stem borer and non-target insects, and showed no obvious abnormality in the microstructure of their digestive tracts. Based on these results, we conclude that cultivation of transgenic cry1Ab/1Ac rice does not adversely affect paddy frogs.
学科主题	Ecology ; Environmental Sciences ; Toxicology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000343877400003
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11855]
专题	植物保护研究所
作者单位	1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China 2.Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 225009, Peoples R China 3.Jiangxi Acad Agr Sci, Inst Plant Protect, Nanchang 330200, Peoples R China
推荐引用方式 GB/T 7714	Wang, Jia-Mei,Chen, Xiu-Ping,Liang, Yu-Yong,et al. Influence of transgenic rice expressing a fused Cry1Ab/1Ac protein on frogs in paddy fields[J]. ECOTOXICOLOGY,2014,23(9):1619-1628.
APA	Wang, Jia-Mei,Chen, Xiu-Ping,Liang, Yu-Yong,Zhu, Hao-Jun,Ding, Jia-Tong,&Peng, Yu-Fa.(2014).Influence of transgenic rice expressing a fused Cry1Ab/1Ac protein on frogs in paddy fields.ECOTOXICOLOGY,23(9),1619-1628.
MLA	Wang, Jia-Mei,et al."Influence of transgenic rice expressing a fused Cry1Ab/1Ac protein on frogs in paddy fields".ECOTOXICOLOGY 23.9(2014):1619-1628.