Real Time PCR Quantification of Sclerotium rolfsii in Chilli Tissue and Soil | |
Gao, Yu-Han1; Miao, Wu2; Guo, Rong-Jun1; Li, Shi-Dong1 | |
刊名 | PLANT PROTECTION SCIENCE |
2015 | |
卷号 | 51期号:2页码:61-66 |
关键词 | chilli root rot specific primer pair pathogen identification population detection population determination |
ISSN号 | 1212-2580 |
DOI | 10.17221/43/2014-PPS |
通讯作者 | Gao, Yu-Han |
英文摘要 | Root rot disease caused by Sclerotium rolfsii is one of the important factors limiting chilli production in southern China. Rapid methods of detection and identification of S. rolfsii are required for disease prediction and management. Herein, we adapted a four-step real time quantitative PCR method to detect S. rolfsii using new designed primer pair S301S/S301A. By using this assay, S. rolfsii was detected in ten soil samples and eight plant samples at concentrations as low as 10(-7) g mycelium/g soil or plant, both with and without typical symptoms, while tissue-culturing method could only detect the fungus in two samples. This rapid and quantitative method is efficient for detection and determination of S. rolfsii population in soil and plants. |
学科主题 | Agronomy ; Plant Sciences |
语种 | 英语 |
出版者 | CZECH ACADEMY AGRICULTURAL SCIENCES |
WOS记录号 | WOS:000354769600002 |
内容类型 | 期刊论文 |
源URL | [http://111.203.20.206/handle/2HMLN22E/11810] |
专题 | 植物保护研究所 |
作者单位 | 1.Chinese Acad Agr Sci, Inst Plant Protect, Key Lab Pest Management Crops, Minist Agr, Beijing 100081, Peoples R China 2.Xiangyan Seed Co, Changsha, Hunan, Peoples R China |
推荐引用方式 GB/T 7714 | Gao, Yu-Han,Miao, Wu,Guo, Rong-Jun,et al. Real Time PCR Quantification of Sclerotium rolfsii in Chilli Tissue and Soil[J]. PLANT PROTECTION SCIENCE,2015,51(2):61-66. |
APA | Gao, Yu-Han,Miao, Wu,Guo, Rong-Jun,&Li, Shi-Dong.(2015).Real Time PCR Quantification of Sclerotium rolfsii in Chilli Tissue and Soil.PLANT PROTECTION SCIENCE,51(2),61-66. |
MLA | Gao, Yu-Han,et al."Real Time PCR Quantification of Sclerotium rolfsii in Chilli Tissue and Soil".PLANT PROTECTION SCIENCE 51.2(2015):61-66. |
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