Identification of heat shock cognate protein 70 gene (Alhsc70) of Apolygus lucorum and its expression in response to different temperature and pesticide stresses

doi:10.1111/1744-7917.12193

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	Identification of heat shock cognate protein 70 gene (Alhsc70) of Apolygus lucorum and its expression in response to different temperature and pesticide stresses
	Sun, Yang 1,2; Zhao, Jing 1; Sheng, Yang 1; Xiao, Ying-Fang 3; Zhang, Yong-Jun 2; Bai, Li-Xin 1; Tan, Yongan 1; Xiao, Liu-Bin 1; Xu, Guang-Chun 1
刊名	INSECT SCIENCE
	2016
卷号	23 期号:1 页码:37-49
关键词	Apolygus lucorum expression files extreme temperature hsc70 pesticide
ISSN号	1672-9609
DOI	10.1111/1744-7917.12193
通讯作者	Sun, Yang
英文摘要	Heat shock cognate protein 70 (Hsc70) is a very important stress-resistance protein of insects against environmental stresses. We employed fluorescent real-time quantitative polymerase chain reaction and Western-blot techniques to analyze the transcriptional and translational expression profiles of AlHSC70 under extreme temperature (4 degrees C and 40 degrees C) or 4 pesticide stresses in Apolygus lucorum. The results showed that the expression of AlHSC70 were significantly induced by cyhalothrin or extremely high temperature (40 degrees C) in both transcriptional and translational levels (P < 0.05), while the transcriptional and translational level of AlHSC70 decreased significantly in treatments of chlorpyrifos or extreme cold temperature (4 degrees C) (P < 0.05). Moreover, after Apolygus lucorum treated by imidacloprid or emamectin benzoate, the expression of AlHSC70 was only up-regulated significantly at the transcriptional level (P < 0.05), although obviously up-regulated at the translational level of AlHSC70. Therefore, this study confirmed that the Alhsc70 gene played important roles in response to both temperature and pesticide stresses, especially for cyhalothrin or extremely high temperature (40 degrees C). In addition, the significant polynomial regression correlations between temperature and the Alhsc70 expression level were shown in all the nymph and adult stages (P < 0.01), indicating temperature was an important factor to affect the relative expression of Alhsc70.
学科主题	Entomology
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000368829000004
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11556]
专题	植物保护研究所
作者单位	1.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Jiangsu, Peoples R China 2.Chinese Acad Agr Sci, Inst Plant Protect, Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China 3.Univ Florida, Mid Florida Res & Educ Ctr, Entomol & Nematol, Apopka, FL USA
推荐引用方式 GB/T 7714	Sun, Yang,Zhao, Jing,Sheng, Yang,et al. Identification of heat shock cognate protein 70 gene (Alhsc70) of Apolygus lucorum and its expression in response to different temperature and pesticide stresses[J]. INSECT SCIENCE,2016,23(1):37-49.
APA	Sun, Yang.,Zhao, Jing.,Sheng, Yang.,Xiao, Ying-Fang.,Zhang, Yong-Jun.,...&Xu, Guang-Chun.(2016).Identification of heat shock cognate protein 70 gene (Alhsc70) of Apolygus lucorum and its expression in response to different temperature and pesticide stresses.INSECT SCIENCE,23(1),37-49.
MLA	Sun, Yang,et al."Identification of heat shock cognate protein 70 gene (Alhsc70) of Apolygus lucorum and its expression in response to different temperature and pesticide stresses".INSECT SCIENCE 23.1(2016):37-49.