Expression pattern of sensory neuron membrane protein(SNMP) in adults of Helicoverpa armigera(Lepidoptera: Noctuidae) and its relation with Gqα

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	Expression pattern of sensory neuron membrane protein(SNMP) in adults of Helicoverpa armigera(Lepidoptera: Noctuidae) and its relation with Gqα
	Su Honghua; Wang Guirong; Guo Yuyuan
刊名	昆虫学报
	2010
卷号	53 期号:8 页码:864-869
关键词	Helicoverpa armigera insect olfaction signal transduction SNMP Gqα yeast two-hybrid technique spatial expression
ISSN号	0454-6296
其他题名	成虫感觉神经元膜蛋白(SNMP)表达及其与Gqα的关系
英文摘要	Insect olfaction is important for insects to detect the food, reproductive partners, oviposition sites, hosts, prey or predators. Sensory neuron membrane protein (SNMP) and Gq alpha subunit (Gqα) were thought to be involved in signal transduction in insect olfaction. In order to make clear the spatial expression pattern of snmp and understand whether Gqα is the direct downstream binding protein of SNMP, semi-quantitative RT-PCR method was used to learn the expression pattern of snmp in antennae, head, thorax, abdomen, legs, wings, proboscis, labial palpus and maxillary palpus of adult Helicoverpa armigera, and yeast two-hybrid technique was used to investigate the relationship between SNMP and Gqα. Semi-quantitative RT-PCR results showed that snmp was expressed not only in the antennae of adult H. armigera, but also in proboscis, labial palpus, maxillary palpus and legs. Yeast two-hybrid screening results showed that Gqα did not directly interact with SNMP. These results suggest that SNMP plays some roles not only in odor detection but also in taste recognition, and SNMP maybe forms a complex with an olfactory receptor (OR) and then binds with Gqα, which needs our further investigation; 昆虫嗅觉对昆虫的取食,求偶,寻找产卵场所,搜寻寄主或猎物至关重要.有研究认为感觉神经元膜蛋白(SNMP)和Gq蛋白α亚基(Gqα)参与信号转导.为了阐明snmp的空间表达情况并确定Gqα是否与SNMP直接结合,我们采用半定量RT-PCR技术对snmp在棉铃虫Helicoverpa armigera(Hbner)成虫的触角,头,胸,腹,足,翅,喙,下颚须和下唇须的分布情况进行了研究,并利用酵母双杂交技术对SNMP与Gqα的关系进行了研究.半定量RT-PCR研究发现, snmp不仅在棉铃虫成虫的触角中表达,而且在喙,下颚须,下唇须及足上都有表达.利用酵母双杂交技术研究发现, Gqα与SNMP不直接相互作用, Gqα不是SNMP的直接下游结合蛋白.这些研究结果说明,SNMP不仅参与气味识别而且也参与味觉识别;SNMP可能与气味受体(OR)形成复合物,然后与Gqα结合,这需要我们进一步深入的研究进行证明
学科主题	昆虫学
语种	英语
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11183]
专题	植物保护研究所
作者单位	Institute of Plant Protection, Chinese Academy of Agricultural Sciences, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing, 100193
推荐引用方式 GB/T 7714	Su Honghua,Wang Guirong,Guo Yuyuan. Expression pattern of sensory neuron membrane protein(SNMP) in adults of Helicoverpa armigera(Lepidoptera: Noctuidae) and its relation with Gqα[J]. 昆虫学报,2010,53(8):864-869.
APA	Su Honghua,Wang Guirong,&Guo Yuyuan.(2010).Expression pattern of sensory neuron membrane protein(SNMP) in adults of Helicoverpa armigera(Lepidoptera: Noctuidae) and its relation with Gqα.昆虫学报,53(8),864-869.
MLA	Su Honghua,et al."Expression pattern of sensory neuron membrane protein(SNMP) in adults of Helicoverpa armigera(Lepidoptera: Noctuidae) and its relation with Gqα".昆虫学报 53.8(2010):864-869.