A Simple Method for the Isolation and Purification of 2,4-Dihydroxy-7-Methoxy-2H-1,4-Benzoxazin-3(4H)-One (DIMBOA) from Maize (Zea mays L.) Seedlings

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	A Simple Method for the Isolation and Purification of 2,4-Dihydroxy-7-Methoxy-2H-1,4-Benzoxazin-3(4H)-One (DIMBOA) from Maize (Zea mays L.) Seedlings
	Li Jing 1; Liu Xingang 1; Dong Fengshou 1; Xu Jun 1; Guo Liqun 1; Kong Zhiqiang 1; TIANYing-ying 1; Wu Yanbin 2; Zheng Yongquan 1
刊名	Journal of Integrative Agriculture
	2013
卷号	12 期号:1 页码:95-102
关键词	DIMBOA maize isolation purification hydroxamic acids
ISSN号	2095-3119
英文摘要	2,4-Dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one (DIMBOA), the dominant benzoxazinoid hydroxamic acid in maize (Zea Mays L.), serves as important factors of resistance against insects and microbial diseases, allelochemicals used in competition with other plants. In this paper, a novel and simple method for the isolation and purification of DIMBOA from maize seedlings was developed. Frozen shoots from 7-d-old maize seedlings (1 000×g) were firstly defrosted and then were directly homogenized and extracted with ethyl acetate. The macerate was allowed to stand at room temperature (25±2)°C for 1 h to allow enzymatic release of DIMBOA from DIMBOA-glucoside. Then the ethyl acetate phase was filtered, dried and evaporated to dryness. The resulting light-tan, semicrystalline residue was stored at -20°C for 24 h. Upon recrystallization from acetone-hexane, a relative higher yield (0.58 g) of pure DIMBOA crystals was obtained compared with the yield afforded by Woodward methodology (0.26 g).
学科主题	农作物
语种	中文
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11147]
专题	植物保护研究所
作者单位	1.Institute of Plant Protection, Chinese Academy of Agricultural Sciences, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing, 100193 2.Henan Institute of Science and Technology, Xinxiang, 453003
推荐引用方式 GB/T 7714	Li Jing,Liu Xingang,Dong Fengshou,et al. A Simple Method for the Isolation and Purification of 2,4-Dihydroxy-7-Methoxy-2H-1,4-Benzoxazin-3(4H)-One (DIMBOA) from Maize (Zea mays L.) Seedlings[J]. Journal of Integrative Agriculture,2013,12(1):95-102.
APA	Li Jing.,Liu Xingang.,Dong Fengshou.,Xu Jun.,Guo Liqun.,...&Zheng Yongquan.(2013).A Simple Method for the Isolation and Purification of 2,4-Dihydroxy-7-Methoxy-2H-1,4-Benzoxazin-3(4H)-One (DIMBOA) from Maize (Zea mays L.) Seedlings.Journal of Integrative Agriculture,12(1),95-102.
MLA	Li Jing,et al."A Simple Method for the Isolation and Purification of 2,4-Dihydroxy-7-Methoxy-2H-1,4-Benzoxazin-3(4H)-One (DIMBOA) from Maize (Zea mays L.) Seedlings".Journal of Integrative Agriculture 12.1(2013):95-102.