Cloning and RNA interference analysis of the salivary protein C002 gene in Schizaphis graminum

doi:10.1016/S2095-3119(14)60822-4

CORC > 中国农业科学院 > 植物保护研究所

	Cloning and RNA interference analysis of the salivary protein C002 gene in Schizaphis graminum
	Zhang Yong; Fan Jia; Sun Jing-rui; Chen Ju-lian
刊名	Journal of Integrative Agriculture
	2015
卷号	14 期号:4 页码:698-705
关键词	Schizaphis graminum salivary protein C002 cDNA clone siRNA
ISSN号	2095-3119
DOI	10.1016/S2095-3119(14)60822-4
英文摘要	The full-length cDNA of functionally-unknown salivary protein C002 in Schizaphis graminum was cloned using rapid amplification of cDNA ends (RACE) and designated as SgC002 (GenBank accession no. KC977563). It is 767 bp long and encodes a protein of 190 amino acid residues with a predicted mass of 21.5 kDa and a predicted cleavage site of N-terminal signal peptide between the 24th and the 25th residues. SgC002 is specifically expressed in salivary gland with the highest level at the 2nd instar. Introducing SgC002-specific 476-siRNA, but not 546-siRNA to aphids through artificial diet significantly suppressed SgC002 expression. Silencing SgC002 gene led to lethality of the aphid on wheat plants, but not on pure artificial diet. Our study demonstrated that artificial diet-mediated RNAi can be a useful tool for research on the roles of genes in aphid salivary gland, and also provided new insights into the characteristics of C002 in wheat aphids.
学科主题	分子生物学
语种	中文
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11082]
专题	植物保护研究所
作者单位	Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Zhang Yong,Fan Jia,Sun Jing-rui,et al. Cloning and RNA interference analysis of the salivary protein C002 gene in Schizaphis graminum[J]. Journal of Integrative Agriculture,2015,14(4):698-705.
APA	Zhang Yong,Fan Jia,Sun Jing-rui,&Chen Ju-lian.(2015).Cloning and RNA interference analysis of the salivary protein C002 gene in Schizaphis graminum.Journal of Integrative Agriculture,14(4),698-705.
MLA	Zhang Yong,et al."Cloning and RNA interference analysis of the salivary protein C002 gene in Schizaphis graminum".Journal of Integrative Agriculture 14.4(2015):698-705.