Identification and molecular mapping of a resistance gene to powdery mildew from the synthetic wheat line M53

doi:10.1007/s13353-010-0006-0

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	Identification and molecular mapping of a resistance gene to powdery mildew from the synthetic wheat line M53
	Li, Tao 1,2; Zhang, Zengyan 1; Hu, Yingkao 3; Duan, Xiayu 4; Xin, Zhiyong 1
刊名	JOURNAL OF APPLIED GENETICS
	2011
卷号	52 期号:2 页码:137-143
关键词	Synthetic wheat Aegilops tauschii Powdery mildew Resistance gene Genetic mapping
ISSN号	1234-1983
DOI	10.1007/s13353-010-0006-0
通讯作者	Li, Tao
英文摘要	Powdery mildew disease caused by Blumeria graminis f. sp. tritici (Bgt) is an economically important disease in wheat worldwide. The identification of germplasms resistant to the disease can not only facilitate the breeding of resistant cultivars, but can also broaden the diversity of resistance genes. The Mexican M53 is a synthetic hexaploid wheat line developed at the International Maize and Wheat Improvement Center (CIMMYT) from the cross between Triticum durum and Aegilops tauschii249. Infection of M53 with 15 different pathogen races revealed that the resistance in M53 was race-dependent and effective against the majority of the tested Bgt races, including the race 15 predominant in the Beijing wheat growing area. Inoculation of the parents of M53 with the race 15 demonstrated that M53 and Ae. tauschii249 were resistant, whereas T. durum was susceptible. The inoculation of three segregating F(2) populations developed from the crosses between M53 and three susceptible Chinese wheat cultivars with the race 15 showed that the resistant gene in M53 segregated in a single dominant manner. Amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers were used to map the gene in a segregating F(2) population consisting of 213 lines developed from the cross Wan7107 x M53. Two closely linked AFLP markers, Apm109 and Apm161, were identified to flank the gene with genetic distances of 1.0 cM and 3.0 cM, respectively. The recognized gene was assigned to the long arm of chromosome 5D as determined by three linked SSR markers, Xwmc289b, Xgwm583, and Xgwm292, and by the physical mapping of Apm109 using Chinese Spring nullisomic-tetrasomic and ditelosomic stocks. The resistance gene identified in M53, temporarily designated as Pm-M53, could be used in local wheat-breeding programs to improve powdery mildew resistance.
学科主题	Biotechnology & Applied Microbiology ; Genetics & Heredity ; BIOTECHNOLOGY & APPLIED MICROBIOLOGY ; GENETICS & HEREDITY
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000290346200003
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5321]
专题	作物科学研究所_其他01
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Key Lab Crop Genet & Breeding, Minist Agr, Beijing 100081, Peoples R China 2.Yangzhou Univ, Minist Educ, Jiangsu Prov Key Lab Crop Genet & Physiol, Key Lab Plant Funct Genom, Yangzhou 225009, Peoples R China 3.Capital Normal Coll, Dept Biol, Beijing 100081, Peoples R China 4.Chinese Acad Agr Sci, Inst Plant Protect, Beijing 100094, Peoples R China
推荐引用方式 GB/T 7714	Li, Tao,Zhang, Zengyan,Hu, Yingkao,et al. Identification and molecular mapping of a resistance gene to powdery mildew from the synthetic wheat line M53[J]. JOURNAL OF APPLIED GENETICS,2011,52(2):137-143.
APA	Li, Tao,Zhang, Zengyan,Hu, Yingkao,Duan, Xiayu,&Xin, Zhiyong.(2011).Identification and molecular mapping of a resistance gene to powdery mildew from the synthetic wheat line M53.JOURNAL OF APPLIED GENETICS,52(2),137-143.
MLA	Li, Tao,et al."Identification and molecular mapping of a resistance gene to powdery mildew from the synthetic wheat line M53".JOURNAL OF APPLIED GENETICS 52.2(2011):137-143.