SLG controls grain size and leaf angle by modulating brassinosteroid homeostasis in rice

doi:10.1093/jxb/erw204

	SLG controls grain size and leaf angle by modulating brassinosteroid homeostasis in rice
	Feng, Zhiming 1; Wu, Chuanyin 2; Wang, Chunming 1; Roh, Jeehee 3; Zhang, Long 1; Chen, Jun 2; Zhang, Shengzhong 1; Zhang, Huan 1; Yang, Chunyan 1; Hu, Jinlong 1
刊名	JOURNAL OF EXPERIMENTAL BOTANY
	2016
卷号	67 期号:14 页码:4241-4253
关键词	BR grain size homomer leaf angle rice SLG
ISSN号	0022-0957
DOI	10.1093/jxb/erw204
通讯作者	Feng, Zhiming
英文摘要	The rice SLG gene, functioning as homomers, plays essential roles in regulating grain size and leaf angle via modulation of brassinosteroid homeostasis.Grain size and leaf angle are two important traits determining grain yield in rice. However, the mechanisms regulating the two traits remain largely unknown. Here, we characterized a rice gain-of-function mutant, slender grain Dominant (slg-D), which exhibited longer and narrower grains and larger leaf angles, similar to plants with elevated brassinosteroid (BR) levels or strengthened BR signaling. The increased cell length is responsible for the mutant phenotypes in slg-D. We demonstrated that the phenotype of slg-D is caused by enhanced expression of SLG, a BAHD acyltransferase-like protein gene. SLG is preferentially expressed in young panicles and lamina joints, implying its role in controlling cell growth in those two tissues. slg-D was restored to wild type by treatment with brassinazole, an inhibitor of BR biosynthesis. Overexpression of SLG in d11-2 (deficient in BR synthesis) and d61-1 (deficient in BR signaling) did not change the existing phenotypes. The slg-D plants had elevated BR contents and, accordingly, expression of BR-related genes was changed in a manner similar to BR treatment. Moreover, SLG RNAi plants displayed mild BR-deficient phenotypes including shorter grains, smaller leaf angles, and compact semi-dwarf plant types. The in vitro biochemical assays and transgenic approaches collectively demonstrated that SLG functions as homomers. Taken together, we conclude that SLG is an important regulator in BR homeostasis and that manipulation of SLG expression to an optimal level may provide a way to develop an ideal plant type.
学科主题	Plant Sciences
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000382060300020
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/105346]
专题	作物科学研究所_转基因中心
作者单位	1.Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Jiangsu Plant Gene Engn Res Ctr, Nanjing 210095, Jiangsu, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Resources & Genet Improvement, Beijing 100081, Peoples R China 3.Chung Ang Univ, Dept Life Sci, Seoul 156756, South Korea 4.Wilkes Univ, Dept Biol, Wilkes Barre, PA 18766 USA
推荐引用方式 GB/T 7714	Feng, Zhiming,Wu, Chuanyin,Wang, Chunming,et al. SLG controls grain size and leaf angle by modulating brassinosteroid homeostasis in rice[J]. JOURNAL OF EXPERIMENTAL BOTANY,2016,67(14):4241-4253.
APA	Feng, Zhiming.,Wu, Chuanyin.,Wang, Chunming.,Roh, Jeehee.,Zhang, Long.,...&Wan, Jianmin.(2016).SLG controls grain size and leaf angle by modulating brassinosteroid homeostasis in rice.JOURNAL OF EXPERIMENTAL BOTANY,67(14),4241-4253.
MLA	Feng, Zhiming,et al."SLG controls grain size and leaf angle by modulating brassinosteroid homeostasis in rice".JOURNAL OF EXPERIMENTAL BOTANY 67.14(2016):4241-4253.