GmPRP2 promoter drives root-preferential expression in transgenic Arabidopsis and soybean hairy roots

doi:10.1186/s12870-014-0245-z

	GmPRP2 promoter drives root-preferential expression in transgenic Arabidopsis and soybean hairy roots
	Chen, Li; Jiang, Bingjun; Wu, Cunxiang; Sun, Shi; Hou, Wensheng; Han, Tianfu
刊名	BMC PLANT BIOLOGY
	2014
卷号	14 页码:-
关键词	Soybean PRP2 Promoter Root-preferential Arabidopsis thaliana Deletion GUS Hairy root
ISSN号	1471-2229
DOI	10.1186/s12870-014-0245-z
通讯作者	Chen, Li
英文摘要	Background: Promoters play important roles in gene expression and function. There are three basic types of promoters: constitutive, specific, and inducible. Constitutive promoters are widely used in genetic engineering, but these promoters have limitations. Inducible promoters are activated by specific inducers. Tissue-specific promoters are a type of specific promoters that drive gene expression in specific tissues or organs. Here, we cloned and characterized the GmPRP2 promoter from soybean. The expression pattern indicated that this promoter is root-preferential in transgenic Arabidopsis and the hairy roots of soybean. It can be used to improve the root resistance or tolerance to pathogens, pests, malnutrition and other abiotic stresses which cause extensive annual losses in soybean production.Results: The GmPRP2 promoter (GmPRP2p-1062) was isolated from soybean cv. Williams 82. Sequence analysis revealed that this promoter contains many cis-acting elements, including root-specific motifs. The GmPRP2p-1062 and its 5'-deletion fragments were fused with the GUS reporter gene and introduced into Arabidopsis and the hairy roots of soybean to further determine promoter activity. Histochemical analysis in transgenic Arabidopsis showed that GUS activity was mainly detected in roots and hypocotyls in all deletion fragments except GmPRP2p-471 (a 5'-deletion fragment of GmPRP2p-1062 with 471 bp length). GUS activity was higher in transgenic Arabidopsis and hairy roots with GmPRP2p-1062 and GmPRP2p-852 (a 5'-deletion fragment of GmPRP2p-1062 with 852 bp length) constructs than the other two constructs. GUS activity was enhanced by NaCl, PEG, IAA and JM treatments and decreased by SA, ABA and GA treatments in transgenic Arabidopsis.Conclusions: GmPRP2p-1062 is a root-preferential promoter, and its core fragment for root-preferential expression might lie between -369 and +1. GmPRP2p-852 may be useful in the genetic engineering of novel soybean cultivars in the future.
学科主题	Plant Sciences
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000342010700001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4840]
专题	作物科学研究所_转基因中心
作者单位	Chinese Acad Agr Sci, Inst Crop Sci, MOA Key Lab Soybean Biol Beijing, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Chen, Li,Jiang, Bingjun,Wu, Cunxiang,et al. GmPRP2 promoter drives root-preferential expression in transgenic Arabidopsis and soybean hairy roots[J]. BMC PLANT BIOLOGY,2014,14:-.
APA	Chen, Li,Jiang, Bingjun,Wu, Cunxiang,Sun, Shi,Hou, Wensheng,&Han, Tianfu.(2014).GmPRP2 promoter drives root-preferential expression in transgenic Arabidopsis and soybean hairy roots.BMC PLANT BIOLOGY,14,-.
MLA	Chen, Li,et al."GmPRP2 promoter drives root-preferential expression in transgenic Arabidopsis and soybean hairy roots".BMC PLANT BIOLOGY 14(2014):-.