Over-expression of phosphoenolpyruvate carboxylase cDNA from C4 millet (Seteria italica) increase rice photosynthesis and yield under upland condition but not in wetland fields | |
Ding, Zai-Song; Huang, Su-Hua; Zhou, Bao-Yuan; Sun, Xue-Fang; Zhao, Ming | |
刊名 | PLANT BIOTECHNOLOGY REPORTS |
2013 | |
卷号 | 7期号:2页码:155-163 |
关键词 | Phosphoenolpyruvate carboxylase Transgenic rice Photosynthesis Yield Drought |
ISSN号 | 1863-5466 |
DOI | 10.1007/s11816-012-0244-1 |
通讯作者 | Ding, Zai-Song |
英文摘要 | Phosphoenolpyruvate carboxylase (PEPC) catalyzes the initial fixation of CO2 in C-4 plants. Under the control of the rice Rubisco small subunit promoter, cDNA of a C-4 SiPPC gene cloned from Seteria italica was introduced into Japonica rice by Agrobacterium-mediated transformation. Integration of the gene was confirmed by PCR analysis. RT-PCR showed expression of the gene at the RNA level in transgenic plants, and enzyme activity measurements confirmed the increase in PEPC protein. The transformants showed improvements in both photosynthesis rate and yield only under upland field cultivation. The possible function of PEPC in rice stress tolerance is discussed. |
学科主题 | Biotechnology & Applied Microbiology ; Plant Sciences ; BIOTECHNOLOGY & APPLIED MICROBIOLOGY ; PLANT SCIENCES |
语种 | 英语 |
出版者 | SPRINGER |
WOS记录号 | WOS:000317858800004 |
内容类型 | 期刊论文 |
源URL | [http://111.203.20.206/handle/2HMLN22E/5050] |
专题 | 作物科学研究所_栽培生理学系 |
作者单位 | Chinese Acad Agr Sci, Inst Crop Sci, Key Lab Crop Physiol & Prod MOA, Beijing 100081, Peoples R China |
推荐引用方式 GB/T 7714 | Ding, Zai-Song,Huang, Su-Hua,Zhou, Bao-Yuan,et al. Over-expression of phosphoenolpyruvate carboxylase cDNA from C4 millet (Seteria italica) increase rice photosynthesis and yield under upland condition but not in wetland fields[J]. PLANT BIOTECHNOLOGY REPORTS,2013,7(2):155-163. |
APA | Ding, Zai-Song,Huang, Su-Hua,Zhou, Bao-Yuan,Sun, Xue-Fang,&Zhao, Ming.(2013).Over-expression of phosphoenolpyruvate carboxylase cDNA from C4 millet (Seteria italica) increase rice photosynthesis and yield under upland condition but not in wetland fields.PLANT BIOTECHNOLOGY REPORTS,7(2),155-163. |
MLA | Ding, Zai-Song,et al."Over-expression of phosphoenolpyruvate carboxylase cDNA from C4 millet (Seteria italica) increase rice photosynthesis and yield under upland condition but not in wetland fields".PLANT BIOTECHNOLOGY REPORTS 7.2(2013):155-163. |
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