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Over-expression of phosphoenolpyruvate carboxylase cDNA from C4 millet (Seteria italica) increase rice photosynthesis and yield under upland condition but not in wetland fields
Ding, Zai-Song; Huang, Su-Hua; Zhou, Bao-Yuan; Sun, Xue-Fang; Zhao, Ming
刊名PLANT BIOTECHNOLOGY REPORTS
2013
卷号7期号:2页码:155-163
关键词Phosphoenolpyruvate carboxylase Transgenic rice Photosynthesis Yield Drought
ISSN号1863-5466
DOI10.1007/s11816-012-0244-1
通讯作者Ding, Zai-Song
英文摘要Phosphoenolpyruvate carboxylase (PEPC) catalyzes the initial fixation of CO2 in C-4 plants. Under the control of the rice Rubisco small subunit promoter, cDNA of a C-4 SiPPC gene cloned from Seteria italica was introduced into Japonica rice by Agrobacterium-mediated transformation. Integration of the gene was confirmed by PCR analysis. RT-PCR showed expression of the gene at the RNA level in transgenic plants, and enzyme activity measurements confirmed the increase in PEPC protein. The transformants showed improvements in both photosynthesis rate and yield only under upland field cultivation. The possible function of PEPC in rice stress tolerance is discussed.
学科主题Biotechnology & Applied Microbiology ; Plant Sciences ; BIOTECHNOLOGY & APPLIED MICROBIOLOGY ; PLANT SCIENCES
语种英语
出版者SPRINGER
WOS记录号WOS:000317858800004
内容类型期刊论文
源URL[http://111.203.20.206/handle/2HMLN22E/5050]  
专题作物科学研究所_栽培生理学系
作者单位Chinese Acad Agr Sci, Inst Crop Sci, Key Lab Crop Physiol & Prod MOA, Beijing 100081, Peoples R China
推荐引用方式
GB/T 7714
Ding, Zai-Song,Huang, Su-Hua,Zhou, Bao-Yuan,et al. Over-expression of phosphoenolpyruvate carboxylase cDNA from C4 millet (Seteria italica) increase rice photosynthesis and yield under upland condition but not in wetland fields[J]. PLANT BIOTECHNOLOGY REPORTS,2013,7(2):155-163.
APA Ding, Zai-Song,Huang, Su-Hua,Zhou, Bao-Yuan,Sun, Xue-Fang,&Zhao, Ming.(2013).Over-expression of phosphoenolpyruvate carboxylase cDNA from C4 millet (Seteria italica) increase rice photosynthesis and yield under upland condition but not in wetland fields.PLANT BIOTECHNOLOGY REPORTS,7(2),155-163.
MLA Ding, Zai-Song,et al."Over-expression of phosphoenolpyruvate carboxylase cDNA from C4 millet (Seteria italica) increase rice photosynthesis and yield under upland condition but not in wetland fields".PLANT BIOTECHNOLOGY REPORTS 7.2(2013):155-163.
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