Map-based cloning and functional analysis of the chromogen gene C in rice (Oryza sativa L.)

doi:10.1007/s12374-016-0227-9

	Map-based cloning and functional analysis of the chromogen gene C in rice (Oryza sativa L.)
	Zhao, Shasha; Wang, Cuihong; Ma, Jian; Wang, Shuai; Tian, Peng; Wang, Jiulin; Cheng, Zhijun; Zhang, Xin; Guo, Xiuping; Lei, Cailin
刊名	JOURNAL OF PLANT BIOLOGY
	2016
卷号	59 期号:5 页码:496-505
关键词	Gene expression Map-based cloning OsC Red apiculus Rice
ISSN号	1226-9239
DOI	10.1007/s12374-016-0227-9
通讯作者	Zhao, Shasha
英文摘要	The chromogen gene C is critical for anthocyanin regulation in rice, and apiculus color is an important agronomic trait in selective breeding and variety purification. Mapbased cloning and in-depth functional analysis of the C gene will be useful for understanding the molecular mechanism of anthocyanin biosynthesis and for rice breeding. Japonica landrace Lijiangxintuanheigu (LTH) has red apiculi and purple stigmas. Genetic analysis showed that red apiculus and purple stigma in LTH co-segregated indicating control by a single dominant gene, or by two completely linked genes. Using 1,851 recessive individuals from two F-2 populations, the target gene OsC was delimited to a 70.8 kb interval on chromosome 6 that contains the rice homologue of the maize anthocyanin regulatory gene C1. When the entire OsC gene and its full-length cDNA cloned from LTH were transformed into japonica cultivar Kitaake with colorless apiculi and stigmas all positive transformants had red apiculi but non-colored stigmas, validating that OsC alone was responsible for the apiculus color and represented the functional C gene. OsC was constitutively expressed in all tissues examined, with strongest expression in leaf blades. These results set a foundation to clarify the regulatory mechanisms of OsC in the anthocyanin biosynthetic pathway.
学科主题	Plant Sciences
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000385424700009
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/105312]
专题	作物科学研究所_分子生物学系
作者单位	Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Shasha,Wang, Cuihong,Ma, Jian,et al. Map-based cloning and functional analysis of the chromogen gene C in rice (Oryza sativa L.)[J]. JOURNAL OF PLANT BIOLOGY,2016,59(5):496-505.
APA	Zhao, Shasha.,Wang, Cuihong.,Ma, Jian.,Wang, Shuai.,Tian, Peng.,...&Lei, Cailin.(2016).Map-based cloning and functional analysis of the chromogen gene C in rice (Oryza sativa L.).JOURNAL OF PLANT BIOLOGY,59(5),496-505.
MLA	Zhao, Shasha,et al."Map-based cloning and functional analysis of the chromogen gene C in rice (Oryza sativa L.)".JOURNAL OF PLANT BIOLOGY 59.5(2016):496-505.