A novel activator-type ERF of Thinopyrum intermedium, TiERF1, positively regulates defence responses

doi:10.1093/jxb/ern165

	A novel activator-type ERF of Thinopyrum intermedium, TiERF1, positively regulates defence responses
	Liang, HongXia; Lu, Yan; Liu, HongXia; Wang, FengDe; Xin, ZhiYong; Zhang, ZengYan
刊名	JOURNAL OF EXPERIMENTAL BOTANY
	2008
卷号	59 期号:11 页码:3111-3120
关键词	ethylene response factor phenotypic change resistance Thinopyrum intermedium transcription activator transgenic tobacco plants
ISSN号	0022-0957
DOI	10.1093/jxb/ern165
通讯作者	Liang, HongXia
英文摘要	Thinopyrum intermedium is resistant to many different pathogens. To understand the roles of ethylene response factors (ERFs) in defence responses, the first member of the ERF family in T. intermedium, TiERF1, was characterized and functionally analysed in this study. The TiERF1 gene encodes a putative protein of 292 amino acids, belonging to the B3 subgroup of the ERF transcription factor family. Biochemical assays demonstrated that the TiERF1 protein is capable of binding to the GCC box, a cis-element present in the promoters of pathogenesis-related (PR) genes, and possessing transactivation activity, as well as localizing to the nucleus. The transcript of TiERF1 in T. intermedium is rapidly induced by infection with Rhizoctonia cerealis, Fusarium graminearum, or Blumeria graminis, and ethylene, jasmonic acid, and salicylic acid treatments. More importantly, the ectopic expression of TiERF1 in tobacco activated the transcript of the PR genes of tobacco with a GCC box cis-element, and ACO and ACS genes key to ethylene synthesis, and in turn improved the resistance level to Alternaria alternata and tobacco mosaic virus, as well as causing some phenotypic changes associated with ethylene response in the transgenic tobacco plants. Taken together, TiERF1 protein as an ERF transcription activator positively regulates defence responses via the activation of some defence-related genes.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000258330200018
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5591]
专题	作物科学研究所_分子生物学系
作者单位	Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Liang, HongXia,Lu, Yan,Liu, HongXia,et al. A novel activator-type ERF of Thinopyrum intermedium, TiERF1, positively regulates defence responses[J]. JOURNAL OF EXPERIMENTAL BOTANY,2008,59(11):3111-3120.
APA	Liang, HongXia,Lu, Yan,Liu, HongXia,Wang, FengDe,Xin, ZhiYong,&Zhang, ZengYan.(2008).A novel activator-type ERF of Thinopyrum intermedium, TiERF1, positively regulates defence responses.JOURNAL OF EXPERIMENTAL BOTANY,59(11),3111-3120.
MLA	Liang, HongXia,et al."A novel activator-type ERF of Thinopyrum intermedium, TiERF1, positively regulates defence responses".JOURNAL OF EXPERIMENTAL BOTANY 59.11(2008):3111-3120.