Dreb1 genes in wheat (Triticum aestivum L.): development of functional markers and gene mapping based on SNPs

doi:10.1007/s11032-008-9209-z

	Dreb1 genes in wheat (Triticum aestivum L.): development of functional markers and gene mapping based on SNPs
	Wei, Bo 1,2; Jing, Ruilian 1; Wang, Chengshe 3; Chen, Jibao 1; Mao, Xinguo 1; Chang, Xiaoping 1; Jia, Jizeng 1
刊名	MOLECULAR BREEDING
	2009
卷号	23 期号:1 页码:13-22
关键词	Wheat Functional marker Single nucleotide polymorphisms Dreb Dehydration-responsive element binding proteins
ISSN号	1380-3743
DOI	10.1007/s11032-008-9209-z
通讯作者	Wei, Bo
英文摘要	Dreb genes are involved in abiotic stress tolerances, such as drought, salinity, low temperature and ABA. The purpose of the present research was to establish protocols for the development of genome-specific and allele specific markers in common wheat ( Triticum aestivum L.) using the Dreb1 genes as an example. Based on the available sequences of Dreb1 genes in common wheat and related species, five primer pairs were designed using Primer Premier 5.0. Two primers, P25F/PR and P21F/P21R, amplified 596- and 1113-bp fragments, respectively, from the A genome, P18F/P18R amplified a 717-bp fragment from the B genome, and primers P22F/PR and P20F/P20R amplified 596- and 1193-bp fragments, respectively, from the D genome. Using these genome-specific primers and the Chinese Spring using nulli-tetrasomic lines, the Dreb1 genes were located on chromosomes 3A, 3B and 3D. Two SNPs (S646 and S770) in Dreb-B1 distinguished the Opata 85 and W7984 parents of the ITMI mapping population, but there was no polymorphism between the orthologous Dreb-A1 and Dreb-D1 sequences. By assaying the genotypes of 115 RILs with the allele-specific primer P40 based on SNP S770, Dreb-B1 was mapped between markers Xmwg818 and Xfbb117 on chromosome 3BL. This genetic mapping of Dreb-B1 on chromosome 3B may be helpful in wheat breeding programs aimed at improving drought tolerance.
学科主题	Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture ; AGRONOMY ; GENETICS & HEREDITY ; HORTICULTURE ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000262268700002
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5552]
专题	作物科学研究所_分子生物学系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Key Lab Crop Germplasm & Biotechnol,Minist Agr, Beijing 100081, Peoples R China 2.Chinese Acad Agr Sci, Grad Sch, Beijing 100081, Peoples R China 3.NW A&F Univ, Coll Agr, Xianyang 712100, Shaanxi, Peoples R China
推荐引用方式 GB/T 7714	Wei, Bo,Jing, Ruilian,Wang, Chengshe,et al. Dreb1 genes in wheat (Triticum aestivum L.): development of functional markers and gene mapping based on SNPs[J]. MOLECULAR BREEDING,2009,23(1):13-22.
APA	Wei, Bo.,Jing, Ruilian.,Wang, Chengshe.,Chen, Jibao.,Mao, Xinguo.,...&Jia, Jizeng.(2009).Dreb1 genes in wheat (Triticum aestivum L.): development of functional markers and gene mapping based on SNPs.MOLECULAR BREEDING,23(1),13-22.
MLA	Wei, Bo,et al."Dreb1 genes in wheat (Triticum aestivum L.): development of functional markers and gene mapping based on SNPs".MOLECULAR BREEDING 23.1(2009):13-22.