Simultaneous selection of major and minor genes: use of QTL to increase selection efficiency of coleoptile length of wheat (Triticum aestivum L.)

doi:10.1007/s00122-009-1017-2

	Simultaneous selection of major and minor genes: use of QTL to increase selection efficiency of coleoptile length of wheat (Triticum aestivum L.)
	Wang, Jiankang 1,2; Chapman, Scott C.3; Bonnett, David G.4; Rebetzke, Greg J.4
刊名	THEORETICAL AND APPLIED GENETICS
	2009
卷号	119 期号:1 页码:65-74
ISSN号	0040-5752
DOI	10.1007/s00122-009-1017-2
通讯作者	Wang, Jiankang
英文摘要	Plant breeders simultaneously select for qualitative traits controlled by one or a small number of major genes, as well as for polygenic traits controlled by multiple genes that may be detected as quantitative trait loci (QTL). In this study, we applied computer simulation to investigate simultaneous selection for alleles at both major and minor gene (as QTL) loci in breeding populations of two wheat parental lines, HM14BS and Sunstate. Loci targeted for selection included six major genes affecting plant height, disease resistance, and grain quality, plus 6 known and 11 "unidentified" QTL affecting coleoptile length (CL). Parental line HM14BS contributed the target alleles at two of the major gene loci, while parental line Sunstate contributed target alleles at four loci. The parents have similar plant height, but HM14BS has a longer coleoptile, a desirable attribute for deep sowing in rainfed environments. Including the wild-type allele at the major reduced-height locus Rht-D1, HM14BS was assumed to have 13 QTL for increased CL, and Sunstate four; these assumptions being derived from mapping studies and empirical data from an actual HM14BS/Sunstate population. Simulation indicated that compared to backcross populations, a single biparental F-1 cross produced the highest frequency of target genotypes (six desired alleles at major genes plus desired QTL alleles for long CL). From 1,000 simulation runs, an average of 2.4 individuals with the target genotype were present in unselected F-1-derived doubled haploid (DH) or recombinant inbred line (RIL) populations of size 200. A selection scheme for the six major genes increased the number of target individuals to 19.1, and additional marker-assisted selection (MAS) for CL increased the number to 23.0. Phenotypic selection (PS) of CL outperformed MAS in this study due to the high heritability of CL, incompletely linked markers for known QTL, and the existence of unidentified QTL. However, a selection scheme combining MAS and PS was equally as efficient as PS and would result in net savings in production and time to delivery of long coleoptile wheats containing the six favorable alleles.
学科主题	Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture ; AGRONOMY ; GENETICS & HEREDITY ; HORTICULTURE ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000266666500007
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5518]
专题	作物科学研究所_分子生物学系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 2.Chinese Acad Agr Sci, CIMMYT China, Beijing 100081, Peoples R China 3.CSIRO Plant Ind, St Lucia, Qld 4067, Australia 4.CSIRO Plant Ind, Canberra, ACT 2601, Australia
推荐引用方式 GB/T 7714	Wang, Jiankang,Chapman, Scott C.,Bonnett, David G.,et al. Simultaneous selection of major and minor genes: use of QTL to increase selection efficiency of coleoptile length of wheat (Triticum aestivum L.)[J]. THEORETICAL AND APPLIED GENETICS,2009,119(1):65-74.
APA	Wang, Jiankang,Chapman, Scott C.,Bonnett, David G.,&Rebetzke, Greg J..(2009).Simultaneous selection of major and minor genes: use of QTL to increase selection efficiency of coleoptile length of wheat (Triticum aestivum L.).THEORETICAL AND APPLIED GENETICS,119(1),65-74.
MLA	Wang, Jiankang,et al."Simultaneous selection of major and minor genes: use of QTL to increase selection efficiency of coleoptile length of wheat (Triticum aestivum L.)".THEORETICAL AND APPLIED GENETICS 119.1(2009):65-74.