Novel microRNAs uncovered by deep sequencing of small RNA transcriptomes in bread wheat (Triticum aestivum L.) and Brachypodium distachyon (L.) Beauv

doi:10.1007/s10142-009-0128-9

	Novel microRNAs uncovered by deep sequencing of small RNA transcriptomes in bread wheat (Triticum aestivum L.) and Brachypodium distachyon (L.) Beauv
	Wei, Bo 1,2; Cai, Tao 3; Zhang, Rongzhi 1,2; Li, Aili 1,2; Huo, Naxin 4; Li, Shan 3; Gu, Yong Q.4; Vogel, John 4; Jia, Jizeng 1,2; Qi, Yijun 3
刊名	FUNCTIONAL & INTEGRATIVE GENOMICS
	2009
卷号	9 期号:4 页码:499-511
关键词	Brachypodium Wheat Small RNA MicroRNA SiRNA
ISSN号	1438-793X
DOI	10.1007/s10142-009-0128-9
通讯作者	Wei, Bo
英文摘要	The small RNA transcriptomes of bread wheat and its emerging model Brachypodium distachyon were obtained by using deep sequencing technology. Small RNA compositions were analyzed in these two species. In addition to 70 conserved microRNAs (miRNAs) from 25 families, 23 novel wheat miRNAs were identified. For Brachypodium, 12 putative miRNAs were predicted from a limited number of expressed sequence tags, of which one was a potential novel miRNA. Also, 94 conserved miRNAs from 28 families were identified in this species. Expression validation was performed for several novel wheat miRNAs. RNA ligase-mediated 5' rapid amplification of complementary DNA ends experiments demonstrated their capability to cleave predicted target genes including three disease-resistant gene analogs. Differential expression of miRNAs was observed between Brachypodium vegetative and reproductive tissues, suggesting their different roles at the two growth stages. Our work significantly increases the novel miRNA numbers in wheat and provides the first set of small RNAs in B. distachyon.
学科主题	Genetics & Heredity ; GENETICS & HEREDITY
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000270633700007
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5479]
专题	作物科学研究所_分子生物学系
作者单位	1.Chinese Acad Agr Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China 3.Natl Inst Biol Sci, Beijing 102206, Peoples R China 4.USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA
推荐引用方式 GB/T 7714	Wei, Bo,Cai, Tao,Zhang, Rongzhi,et al. Novel microRNAs uncovered by deep sequencing of small RNA transcriptomes in bread wheat (Triticum aestivum L.) and Brachypodium distachyon (L.) Beauv[J]. FUNCTIONAL & INTEGRATIVE GENOMICS,2009,9(4):499-511.
APA	Wei, Bo.,Cai, Tao.,Zhang, Rongzhi.,Li, Aili.,Huo, Naxin.,...&Mao, Long.(2009).Novel microRNAs uncovered by deep sequencing of small RNA transcriptomes in bread wheat (Triticum aestivum L.) and Brachypodium distachyon (L.) Beauv.FUNCTIONAL & INTEGRATIVE GENOMICS,9(4),499-511.
MLA	Wei, Bo,et al."Novel microRNAs uncovered by deep sequencing of small RNA transcriptomes in bread wheat (Triticum aestivum L.) and Brachypodium distachyon (L.) Beauv".FUNCTIONAL & INTEGRATIVE GENOMICS 9.4(2009):499-511.