Overexpression of a PLD alpha 1 gene from Setaria italica enhances the sensitivity of Arabidopsis to abscisic acid and improves its drought tolerance

doi:10.1007/s00299-010-0865-1

	Overexpression of a PLD alpha 1 gene from Setaria italica enhances the sensitivity of Arabidopsis to abscisic acid and improves its drought tolerance
	Peng, Yunling 2,3; Zhang, Jinpeng 1; Cao, Gaoyi 4; Xie, Yuanhong 1; Liu, Xihui 4; Lu, Minhui 4; Wang, Guoying 1
刊名	PLANT CELL REPORTS
	2010
卷号	29 期号:7 页码:793-802
关键词	Setaria italica PLD alpha 1 ABA sensitivity Drought tolerance
ISSN号	0721-7714
DOI	10.1007/s00299-010-0865-1
通讯作者	Peng, Yunling
英文摘要	Phospholipase D (PLD) plays an important role in various physiological processes in plants, including drought tolerance. Here, we report the cloning and characterization of the full-length cDNA of PLD alpha 1 from foxtail millet, which is a cereal crop with high water use efficiency. The expression pattern of the SiPLD alpha 1 gene in foxtail millet revealed that it is up-regulated under dehydration, ABA and NaCl treatments. Heterologous overexpression of SiPLD alpha 1 in Arabidopsis can significantly enhance their sensitivity to ABA, NaCl and mannitol during post-germination growth. Under water deprivation, overexpression of SiPLD alpha 1 in Arabidopsis resulted in significantly enhanced tolerance to drought stress, displaying higher biomass and RWC, lower ion leakage and higher survival percentages than the wild type. Further analysis indicated that transgenic plants showed increased transcription of the stress-related genes, RD29A, RD29B, RAB18 and RD22, and the ABA-related genes, ABI1 and NCED3 under dehydration conditions. These results demonstrate that SiPLD alpha 1 is involved in plant stress signal transduction, especially in the ABA signaling pathway. Moreover, no obvious adverse effects on growth and development in the 35S::SiPLD alpha 1 transgenic plants implied that SiPLD alpha 1 is a good candidate gene for improving crop drought tolerance.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000278838400012
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5412]
专题	作物科学研究所_分子生物学系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China 2.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China 3.Gansu Agr Univ, Coll Agron, Lanzhou 730070, Peoples R China 4.China Agr Univ, Coll Agron & Biotechnol, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Peng, Yunling,Zhang, Jinpeng,Cao, Gaoyi,et al. Overexpression of a PLD alpha 1 gene from Setaria italica enhances the sensitivity of Arabidopsis to abscisic acid and improves its drought tolerance[J]. PLANT CELL REPORTS,2010,29(7):793-802.
APA	Peng, Yunling.,Zhang, Jinpeng.,Cao, Gaoyi.,Xie, Yuanhong.,Liu, Xihui.,...&Wang, Guoying.(2010).Overexpression of a PLD alpha 1 gene from Setaria italica enhances the sensitivity of Arabidopsis to abscisic acid and improves its drought tolerance.PLANT CELL REPORTS,29(7),793-802.
MLA	Peng, Yunling,et al."Overexpression of a PLD alpha 1 gene from Setaria italica enhances the sensitivity of Arabidopsis to abscisic acid and improves its drought tolerance".PLANT CELL REPORTS 29.7(2010):793-802.