Cloning and characterization of the stress-induced bZIP gene ZmbZIP60 from maize

doi:10.1007/s11033-012-1453-y

	Cloning and characterization of the stress-induced bZIP gene ZmbZIP60 from maize
	Wang, Bo 1,2; Zheng, Jun 2; Liu, Yunjun 2; Wang, Jianhua 1; Wang, Guoying 1,2
刊名	MOLECULAR BIOLOGY REPORTS
	2012
卷号	39 期号:5 页码:6319-6327
关键词	Maize ZmbZIP60 ER stress Floral transition DTT
ISSN号	0301-4851
DOI	10.1007/s11033-012-1453-y
通讯作者	Wang, Bo
英文摘要	ZmbZIP60 is a member of the bZIP transcription factor family in maize. Expression of ZmbZIP60 is strongly induced by a wide spectrum of stresses, including dehydration, high salinity, abscisic acid and tunicamycin treatments. A truncated form of ZmbZIP60, without a transmembrane domain (ZmbZIP60 Delta C) and fused with GFP, is localized in the nucleus, suggesting the translocation of the native protein to the nucleus by release from the membrane. Yeast one-hybrid analysis showed that both ZmbZIP60 and ZmbZIP60 Delta C had transcriptional activity. The expression of ZmbZIP60 in Arabidopsis bzip60 mutant partially restored the induction of BiP3 transcription under TM treatment, which indicated that ZmbZIP60 may play a role in the signal transduction of endoplasmic reticulum stress. Overexpression of ZmbZIP60 in wild-type Arabidopsis displayed enhanced bolting trends when subjected to dithiothreitol stress. Real-time PCR analysis revealed that some key genes in floral transition, including CO, FT, and AP1, were up- or down-regulated in ZmbZIP60-overexpressing plants, which may reveal the functional difference of ZmbZIP60 from AtbZIP60.
学科主题	Biochemistry & Molecular Biology ; BIOCHEMISTRY & MOLECULAR BIOLOGY
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000302147800149
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5165]
专题	作物科学研究所_分子生物学系
作者单位	1.China Agr Univ, Dept Seed Sci & Technol, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Wang, Bo,Zheng, Jun,Liu, Yunjun,et al. Cloning and characterization of the stress-induced bZIP gene ZmbZIP60 from maize[J]. MOLECULAR BIOLOGY REPORTS,2012,39(5):6319-6327.
APA	Wang, Bo,Zheng, Jun,Liu, Yunjun,Wang, Jianhua,&Wang, Guoying.(2012).Cloning and characterization of the stress-induced bZIP gene ZmbZIP60 from maize.MOLECULAR BIOLOGY REPORTS,39(5),6319-6327.
MLA	Wang, Bo,et al."Cloning and characterization of the stress-induced bZIP gene ZmbZIP60 from maize".MOLECULAR BIOLOGY REPORTS 39.5(2012):6319-6327.