A Novel 5-Enolpyruvylshikimate-3-Phosphate Synthase Shows High Glyphosate Tolerance in Escherichia coli and Tobacco Plants

doi:10.1371/journal.pone.0038718

	A Novel 5-Enolpyruvylshikimate-3-Phosphate Synthase Shows High Glyphosate Tolerance in Escherichia coli and Tobacco Plants
	Cao, Gaoyi 1,2; Liu, Yunjun 2; Zhang, Shengxue 2; Yang, Xuewen 2; Chen, Rongrong 2; Zhang, Yuwen 1; Lu, Wei 3; Liu, Yan 2; Wang, Jianhua 1; Lin, Min 3
刊名	PLOS ONE
	2012
卷号	7 期号:6 页码:-
ISSN号	1932-6203
DOI	10.1371/journal.pone.0038718
通讯作者	Cao, Gaoyi
英文摘要	A key enzyme in the shikimate pathway, 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) is the primary target of the broad-spectrum herbicide glyphosate. Identification of new aroA genes coding for EPSPS with a high level of glyphosate tolerance is essential for the development of glyphosate-tolerant crops. In the present study, the glyphosate tolerance of five bacterial aroA genes was evaluated in the E. coli aroA-defective strain ER2799 and in transgenic tobacco plants. All five aroA genes could complement the aroA-defective strain ER2799, and AM79 aroA showed the highest glyphosate tolerance. Although glyphosate treatment inhibited the growth of both WT and transgenic tobacco plants, transgenic plants expressing AM79 aroA tolerated higher concentration of glyphosate and had a higher fresh weight and survival rate than plants expressing other aroA genes. When treated with high concentration of glyphosate, lower shikimate content was detected in the leaves of transgenic plants expressing AM79 aroA than transgenic plants expressing other aroA genes. These results suggest that AM79 aroA could be a good candidate for the development of transgenic glyphosate-tolerant crops.
学科主题	Multidisciplinary Sciences ; MULTIDISCIPLINARY SCIENCES
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000305336800050
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5154]
专题	作物科学研究所_分子生物学系
作者单位	1.China Agr Univ, Coll Agr & Biotechnol, Beijing 100094, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100193, Peoples R China 3.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Cao, Gaoyi,Liu, Yunjun,Zhang, Shengxue,et al. A Novel 5-Enolpyruvylshikimate-3-Phosphate Synthase Shows High Glyphosate Tolerance in Escherichia coli and Tobacco Plants[J]. PLOS ONE,2012,7(6):-.
APA	Cao, Gaoyi.,Liu, Yunjun.,Zhang, Shengxue.,Yang, Xuewen.,Chen, Rongrong.,...&Wang, Guoying.(2012).A Novel 5-Enolpyruvylshikimate-3-Phosphate Synthase Shows High Glyphosate Tolerance in Escherichia coli and Tobacco Plants.PLOS ONE,7(6),-.
MLA	Cao, Gaoyi,et al."A Novel 5-Enolpyruvylshikimate-3-Phosphate Synthase Shows High Glyphosate Tolerance in Escherichia coli and Tobacco Plants".PLOS ONE 7.6(2012):-.