Molecular characterization of LMW-GS genes in Brachypodium distachyon L. reveals highly conserved Glu-3 loci in Triticum and related species

doi:10.1186/1471-2229-12-221

	Molecular characterization of LMW-GS genes in Brachypodium distachyon L. reveals highly conserved Glu-3 loci in Triticum and related species
	Wang, Shunli 1; Wang, Ke 2; Chen, Guanxing 1; Lv, Dongwen 1; Han, Xiaofeng 1; Yu, Zitong 1; Li, Xiaohui 1; Ye, Xingguo 2; Hsam, S. L. K.3; Ma, Wujun 4,5
刊名	BMC PLANT BIOLOGY
	2012
卷号	12 页码:-
ISSN号	1471-2229
DOI	10.1186/1471-2229-12-221
通讯作者	Wang, Shunli
英文摘要	Background: Brachypodium distachyon L. is a newly emerging model plant system for temperate cereal crop species. However, its grain protein compositions are still not clear. In the current study, we carried out a detailed proteomics and molecular genetics study on grain glutenin proteins in B. distachyon.Results: SDS-PAGE and RP-HPLC analysis of grain proteins showed that Brachypodium has few gliadins and high molecular weight glutenin subunits. In contrast the electrophoretic patterns for the albumin, globulin and low molecular weight glutenin subunit (LMW-GS) fractions of the grain protein were similar to those in wheat. In particular, the LMW-C type subunits in Brachypodium were more abundant than the equivalent proteins in common wheat. Southern blotting analysis confirmed that Brachypodium has 4-5 copies of LMW-GS genes. A total of 18 LMW-GS genes were cloned from Brachypodium by allele specific PCR. LMW-GS and 4 deduced amino acid sequences were further confirmed by using Western-blotting and MALDI-TOF-MS. Phylogenetic analysis indicated that Brachypodium was closer to Ae. markgrafii and Ae. umbellulata than to T. aestivum.Conclusions: Brachypodium possessed a highly conserved Glu-3 locus that is closely related to Triticum and related species. The presence of LMW-GS in B. distachyon grains indicates that B. distachyon may be used as a model system for studying wheat quality attributes.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000313690000001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5113]
专题	作物科学研究所_分子生物学系
作者单位	1.Capital Normal Univ, Coll Life Sci, Key Lab Genet & Biotechnol, Beijing 100048, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 3.Tech Univ Munich, Div Plant Breeding & Appl Genet, D-85350 Freising Weihenstephan, Germany 4.Murdoch Univ, State Agr Biotechnol Ctr, Perth, WA 6150, Australia 5.Western Australian Dept Agr & Food, Perth, WA 6150, Australia
推荐引用方式 GB/T 7714	Wang, Shunli,Wang, Ke,Chen, Guanxing,et al. Molecular characterization of LMW-GS genes in Brachypodium distachyon L. reveals highly conserved Glu-3 loci in Triticum and related species[J]. BMC PLANT BIOLOGY,2012,12:-.
APA	Wang, Shunli.,Wang, Ke.,Chen, Guanxing.,Lv, Dongwen.,Han, Xiaofeng.,...&Yan, Yueming.(2012).Molecular characterization of LMW-GS genes in Brachypodium distachyon L. reveals highly conserved Glu-3 loci in Triticum and related species.BMC PLANT BIOLOGY,12,-.
MLA	Wang, Shunli,et al."Molecular characterization of LMW-GS genes in Brachypodium distachyon L. reveals highly conserved Glu-3 loci in Triticum and related species".BMC PLANT BIOLOGY 12(2012):-.