Cloning and characterization of TaSnRK2.3, a novel SnRK2 gene in common wheat

doi:10.1093/jxb/ert072

	Cloning and characterization of TaSnRK2.3, a novel SnRK2 gene in common wheat
	Tian, Shanjun 1,2; Mao, Xinguo 1; Zhang, Hongying 1; Chen, Shuangshuang 1; Zhai, Chaochao 1; Yang, Shimin 2; Jing, Ruilian 1
刊名	JOURNAL OF EXPERIMENTAL BOTANY
	2013
卷号	64 期号:7 页码:2063-2080
关键词	expression pattern morphological character osmotic stress physiological trait stress response
ISSN号	0022-0957
DOI	10.1093/jxb/ert072
通讯作者	Tian, Shanjun
英文摘要	Environmental stresses such as drought, salinity, and cold are major adverse factors that significantly affect agricultural productivity. Protein phosphorylation/dephosphorylation is a major signalling event induced by osmotic stress in higher plants. Sucrose non-fermenting 1-related protein kinase 2 (SnRK2) family members play essential roles in the response to hyperosmotic stresses in plants. In this study, the TaSnRK2.3 gene, a novel SnRK2 member was cloned, and three copies located on chromosomes 1A, 1B, and 1D were identified in common wheat. TaSnRK2.3 was strongly expressed in leaves, and responded to polyethylene glycol, NaCl, abscisic acid, and cold stresses. To characterize its function, transgenic Arabidopsis overexpressing TaSnRK2.3GFP controlled by the cauliflower mosaic virus 35S promoter was generated and subjected to severe abiotic stresses. Overexpression of TaSnRK2.3 resulted in an improved root system and significantly enhanced tolerance to drought, salt, and freezing stresses, simultaneously demonstrated by enhanced expression of abiotic stress-responsive genes and ameliorative physiological indices, including a decreased rate of water loss, enhanced cell membrane stability, improved photosynthetic potential, and significantly increased osmotic potential and free proline content under normal and/or stressed conditions. These results demonstrate that TaSnRK2.3 is a multifunctional regulator, with potential for utilization in transgenic breeding for improved abiotic stress tolerance in crop plants.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000318651400024
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5051]
专题	作物科学研究所_分子生物学系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Key Lab Crop Germplasm Resource & Enhancement, MOA,Natl Key Facil Crop Gene Resources & Genet Im, Beijing 100081, Peoples R China 2.Sichuan Agr Univ, Coll Agron, Yaan 625014, Sichuan, Peoples R China
推荐引用方式 GB/T 7714	Tian, Shanjun,Mao, Xinguo,Zhang, Hongying,et al. Cloning and characterization of TaSnRK2.3, a novel SnRK2 gene in common wheat[J]. JOURNAL OF EXPERIMENTAL BOTANY,2013,64(7):2063-2080.
APA	Tian, Shanjun.,Mao, Xinguo.,Zhang, Hongying.,Chen, Shuangshuang.,Zhai, Chaochao.,...&Jing, Ruilian.(2013).Cloning and characterization of TaSnRK2.3, a novel SnRK2 gene in common wheat.JOURNAL OF EXPERIMENTAL BOTANY,64(7),2063-2080.
MLA	Tian, Shanjun,et al."Cloning and characterization of TaSnRK2.3, a novel SnRK2 gene in common wheat".JOURNAL OF EXPERIMENTAL BOTANY 64.7(2013):2063-2080.