Characterization of a Maize Wip1 Promoter in Transgenic Plants

doi:10.3390/ijms141223872

	Characterization of a Maize Wip1 Promoter in Transgenic Plants
	Zhang, Shengxue 1; Lian, Yun 2; Liu, Yan 1; Wang, Xiaoqing 1; Liu, Yunjun 1; Wang, Guoying 1
刊名	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
	2013
卷号	14 期号:12 页码:23872-23892
关键词	Wip1 promoter transcriptional start site transgenic plant
ISSN号	1422-0067
DOI	10.3390/ijms141223872
通讯作者	Zhang, Shengxue
英文摘要	The Maize Wip1 gene encodes a wound-induced Bowman-Birk inhibitor (BBI) protein which is a type of serine protease inhibitor, and its expression is induced by wounding or infection, conferring resistance against pathogens and pests. In this study, the maize Wip1 promoter was isolated and its function was analyzed. Different truncated Wip1 promoters were fused upstream of the GUS reporter gene and transformed into Arabidopsis, tobacco and rice plants. We found that (1) several truncated maize Wip1 promoters led to strong GUS activities in both transgenic Arabidopsis and tobacco leaves, whereas low GUS activity was detected in transgenic rice leaves; (2) the Wip1 promoter was not wound-induced in transgenic tobacco leaves, but was induced by wounding in transgenic rice leaves; (3) the truncated Wip1 promoter had different activity in different organs of transgenic tobacco plants; (4) the transgenic plant leaves containing different truncated Wip1 promoters had low GUS transcripts, even though high GUS protein level and GUS activities were observed; (5) there was one transcription start site of Wip1 gene in maize and two transcription start sites of GUS in Wip1::GUS transgenic lines; (6) the adjacent 35S promoter which is present in the transformation vectors enhanced the activity of the truncated Wip1 promoters in transgenic tobacco leaves, but did not influence the disability of truncated Wip(1231) promoter to respond to wounding signals. We speculate that an ACAAAA hexamer, several CAA trimers and several elements similar to ACAATTAC octamer in the 5-untranslated region might contribute to the strong GUS activity in Wip(1231) transgenic lines, meanwhile, compared to the 5-untranslated region from Wip(1231) transgenic lines, the additional upstream open reading frames (uORFs) in the 5-untranslated region from Wip(1737) transgenic lines might contribute to the lower level of GUS transcript and GUS activity.
学科主题	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary ; CHEMISTRY, MULTIDISCIPLINARY
语种	英语
出版者	MDPI AG
WOS记录号	WOS:000330219800045
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4979]
专题	作物科学研究所_分子生物学系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China 2.Henan Acad Agr Sci, Inst Ind Crops, Zhengzhou 450002, Henan, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Shengxue,Lian, Yun,Liu, Yan,et al. Characterization of a Maize Wip1 Promoter in Transgenic Plants[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2013,14(12):23872-23892.
APA	Zhang, Shengxue,Lian, Yun,Liu, Yan,Wang, Xiaoqing,Liu, Yunjun,&Wang, Guoying.(2013).Characterization of a Maize Wip1 Promoter in Transgenic Plants.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,14(12),23872-23892.
MLA	Zhang, Shengxue,et al."Characterization of a Maize Wip1 Promoter in Transgenic Plants".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 14.12(2013):23872-23892.