Production and identification of haploid dwarf male sterile wheat plants induced by corn inducer

doi:10.1186/1999-3110-55-26

	Production and identification of haploid dwarf male sterile wheat plants induced by corn inducer
	Zhang, Wei; Wang, Ke; Lin, Zhi-Shan; Du, Li-Pu; Ma, Hai-Li; Xiao, Le-Le; Ye, Xing-Guo
刊名	BOTANICAL STUDIES
	2014
卷号	55 页码:-
关键词	Corn inducer Dwarf male sterile wheat Intergeneric cross Ms2 haploid plant
ISSN号	1999-3110
DOI	10.1186/1999-3110-55-26
通讯作者	Zhang, Wei
英文摘要	Background: Using the cross of wheat and maize is a very useful way to produce wheat haploid plants by chromosome elimination. Dwarf male sterile wheat (DMSW) and corn inducer are potential important germplasm for wheat breeding by recurrent selection and doubled haploid strategies. There is no report yet to achieve the haploid plants from DMSW induced by maize inbred line and especially the corn inducer.Results: Haploid plants of DMSW were successfully obtained in this study induced by both maize pollens of inducer line and normal inbred line. The efficiencies for wheat embryos formation and plantlets production induced by the two corn lines had no significant difference. All the eleven haploid wheat plants derived from the male sterile material were identified by botanic appearance, cytology, cytogenetics, and molecular markers. They were all haploid based on their guard cell length of 42.78-42.90 mu m compared with the diploid control of 71.52 mu m, and their chromosome number of 21 compared with the diploid control of 42. In addition, according to anthers, plant height, and molecular markers, the haploid plants were divided into two types. Eight of them showed dwarf, having no anthers, and the special band of Rht10, and the other three plants displayed normal plant height, having anthers, and not containing the special band of Rht10, indicating that they were originated from the MS2/Rht10 and ms2/rht10 female gametes, respectively.Conclusions: MS2/Rht10 haploid plants were successfully obtained in this study by using corn inducer and inbred line, and will be employed as candidate materials for the potential cloning of MS2 dominant male gene.
学科主题	Plant Sciences
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000347308500001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4926]
专题	作物科学研究所_分子生物学系
作者单位	Chinese Acad Agr Sci, Inst Crop Sci,Minist Agr, Natl Key Facil Crop Gene Resources & Genet Improv, Key Lab Biol & Genet Improvement Triticeae Crops, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Wei,Wang, Ke,Lin, Zhi-Shan,et al. Production and identification of haploid dwarf male sterile wheat plants induced by corn inducer[J]. BOTANICAL STUDIES,2014,55:-.
APA	Zhang, Wei.,Wang, Ke.,Lin, Zhi-Shan.,Du, Li-Pu.,Ma, Hai-Li.,...&Ye, Xing-Guo.(2014).Production and identification of haploid dwarf male sterile wheat plants induced by corn inducer.BOTANICAL STUDIES,55,-.
MLA	Zhang, Wei,et al."Production and identification of haploid dwarf male sterile wheat plants induced by corn inducer".BOTANICAL STUDIES 55(2014):-.