Expression of Wheat High Molecular Weight Glutenin Subunit 1Bx Is Affected by Large Insertions and Deletions Located in the Upstream Flanking Sequences

doi:10.1371/journal.pone.0105363

	Expression of Wheat High Molecular Weight Glutenin Subunit 1Bx Is Affected by Large Insertions and Deletions Located in the Upstream Flanking Sequences
	Geng, Yuke 1,2; Pang, Binshuang 3; Hao, Chenyang 1; Tang, Saijun 2; Zhang, Xueyong 1; Li, Tian 1
刊名	PLOS ONE
	2014
卷号	9 期号:8 页码:-
ISSN号	1932-6203
DOI	10.1371/journal.pone.0105363
通讯作者	Geng, Yuke
英文摘要	To better understand the transcriptional regulation of high molecular weight glutenin subunit (HMW-GS) expression, we isolated four Glu-1Bx promoters from six wheat cultivars exhibiting diverse protein expression levels. The activities of the diverse Glu-1Bx promoters were tested and compared with beta-glucuronidase (GUS) reporter fusions. Although all the full-length Glu-1Bx promoters showed endosperm-specific activities, the strongest GUS activity was observed with the 1Bx7(OE) promoter in both transient expression assays and stable transgenic rice lines. A 43 bp insertion in the 1Bx7(OE) promoter, which is absent in the 1Bx7 promoter, led to enhanced expression. Analysis of promoter deletion constructs confirmed that a 185 bp MITE (miniature inverted-repeat transposable element) in the 1Bx14 promoter had a weak positive effect on Glu-1Bx expression, and a 54 bp deletion in the 1Bx13 promoter reduced endosperm-specific activity. To investigate the effect of the 43 bp insertion in the 1Bx7(OE) promoter, a functional marker was developed to screen 505 Chinese varieties and 160 European varieties, and only 1Bx7-type varieties harboring the 43 bp insertion in their promoters showed similar overexpression patterns. Hence, the 1Bx7(OE) promoter should be important tool in crop genetic engineering as well as in molecular assisted breeding.
学科主题	Multidisciplinary Sciences
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000341302700104
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4849]
专题	作物科学研究所_分子生物学系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Key Lab Crop Gene Resources & Germplasm Enhanceme, Minist Agr, Beijing 100193, Peoples R China 2.China Agr Univ, Coll Biol Sci, Beijing 100094, Peoples R China 3.Beijing Acad Agr & Forestry Sci, Beijing Engn & Tech Res Ctr Hybrid Wheat, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Geng, Yuke,Pang, Binshuang,Hao, Chenyang,et al. Expression of Wheat High Molecular Weight Glutenin Subunit 1Bx Is Affected by Large Insertions and Deletions Located in the Upstream Flanking Sequences[J]. PLOS ONE,2014,9(8):-.
APA	Geng, Yuke,Pang, Binshuang,Hao, Chenyang,Tang, Saijun,Zhang, Xueyong,&Li, Tian.(2014).Expression of Wheat High Molecular Weight Glutenin Subunit 1Bx Is Affected by Large Insertions and Deletions Located in the Upstream Flanking Sequences.PLOS ONE,9(8),-.
MLA	Geng, Yuke,et al."Expression of Wheat High Molecular Weight Glutenin Subunit 1Bx Is Affected by Large Insertions and Deletions Located in the Upstream Flanking Sequences".PLOS ONE 9.8(2014):-.