Novel MiRNA and PhasiRNA Biogenesis Networks in Soybean Roots from Two Sister Lines That Are Resistant and Susceptible to SCN Race 4

doi:10.1371/journal.pone.0110051

	Novel MiRNA and PhasiRNA Biogenesis Networks in Soybean Roots from Two Sister Lines That Are Resistant and Susceptible to SCN Race 4
	Xu, Miaoyun 1; Li, Yinghui 2; Zhang, Qiuxue 1; Xu, Tao 1; Qiu, Lijuan 2; Fan, Yunliu 1; Wang, Lei 1
刊名	PLOS ONE
	2014
卷号	9 期号:10 页码:-
ISSN号	1932-6203
DOI	10.1371/journal.pone.0110051
通讯作者	Xu, Miaoyun
英文摘要	The soybean cyst nematode (SCN), Heterodera glycines, is the most devastating pathogen of soybean worldwide. SiRNAs (small interfere RNAs) have been proven to induce the silencing of cyst nematode genes. However, whether small RNAs from soybean root have evolved a similar mechanism against SCN is unknown. Two genetically related soybean sister lines (ZP03-5373 and ZP03-5413), which are resistant and susceptible, respectively, to SCN race 4 infection were selected for small RNA deep sequencing to identify small RNAs targeted to SCN. We identified 71 less-conserved miRNAs-miRNAs* counterparts belonging to 32 families derived from 91 loci, and 88 novel soybean-specific miRNAs with distinct expression patterns. The identified miRNAs targeted 42 genes representing a wide range of enzymatic and regulatory activities. Roots of soybean conserved one TAS (Trans-acting siRNA) gene family with a similar but unique trans- acting small interfering RNA (tasiRNA) biogenesis profile. In addition, we found that six miRNAs (gma-miR393, 1507, 1510, 1515, 171, 2118) guide targets to produce secondary phasiRNAs (phased, secondary, small interfering RNAs) in soybean root. Multiple targets of these phasiRNAs were predicted and detected. Importantly, we also found that the expression of 34 miRNAs differed significantly between the two lines. Seven ZP03-5373-specific miRNAs were differentially expressed after SCN infection. Forty-four transcripts from SCN were predicted to be potential targets of ZP03-5373-specific differential miRNAs. These findings suggest that miRNAs play an important role in the soybean response to SCN.
学科主题	Multidisciplinary Sciences
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000346765000011
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4820]
专题	作物科学研究所_分子生物学系
作者单位	1.Chinese Acad Agr Sci, Biotechnol Res Inst, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100193, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Xu, Miaoyun,Li, Yinghui,Zhang, Qiuxue,et al. Novel MiRNA and PhasiRNA Biogenesis Networks in Soybean Roots from Two Sister Lines That Are Resistant and Susceptible to SCN Race 4[J]. PLOS ONE,2014,9(10):-.
APA	Xu, Miaoyun.,Li, Yinghui.,Zhang, Qiuxue.,Xu, Tao.,Qiu, Lijuan.,...&Wang, Lei.(2014).Novel MiRNA and PhasiRNA Biogenesis Networks in Soybean Roots from Two Sister Lines That Are Resistant and Susceptible to SCN Race 4.PLOS ONE,9(10),-.
MLA	Xu, Miaoyun,et al."Novel MiRNA and PhasiRNA Biogenesis Networks in Soybean Roots from Two Sister Lines That Are Resistant and Susceptible to SCN Race 4".PLOS ONE 9.10(2014):-.