G-protein beta subunit AGB1 positively regulates salt stress tolerance in Arabidopsis

doi:10.1016/82095-3119(14)60777-2

	G-protein beta subunit AGB1 positively regulates salt stress tolerance in Arabidopsis
	Ma Ya-nan 1,2; Chen Ming 2; Xu Dong-bei 1; Fang Guang-ning 1; Wang Er-hui 1; Gao Shi-qing 3; Xu Zhao-shi 2; Li Lian-cheng 2; Zhang Xiao-hong 1; Min Dong-hong 1
刊名	JOURNAL OF INTEGRATIVE AGRICULTURE
	2015
卷号	14 期号:2 页码:314-325
关键词	Arabidopsis heterotrimeric G-protein beta subunit physiological processes salt stress tolerance
ISSN号	2095-3119
DOI	10.1016/82095-3119(14)60777-2
通讯作者	Ma Ya-nan
英文摘要	The heterotrimeric GTP-binding proteins (G-proteins) in eukaryotes consisted of alpha, beta and gamma subunits and are important in molecular signaling by interacting with G-protein-coupled receptors (GPCR), on which to transduce signaling into the cytoplast through appropriate downstream effectors. However, downstream effectors regulated by the G-proteins in plants are currently not well defined. In this study, the transcripts of AGB1, a G protein 13 subunit gene in Arabidopsis were found to be down-regulated by cold and heat, but up-regulated by high salt stress treatment. AGB1 mutant (agb1-2) was more sensitive to high salt stress than wild-type (WT). Compared with WT, the cotyledon greening rates, fresh weight, root length, seedling germination rates and survival rates decreased more rapidly in agb1-2 along with increasing concentrations of NaCI in normal (MS) medium. Physiological characteristic analysis showed that compared to WT, the contents of chlorophyll, relative proline accumulation and peroxidase (POD) were reduced, whereas the malonaldehyde (MDA) content and concentration ratio of Na+/K+ were increased in agb1-2 under salt stress condition. Further studies on the expression of several stress inducible genes associated with above physiological processes were investigated, and the results revealed that the expressions of genes related to praline biosynthesis, oxidative stress response, Na+ homeostasis, stress- and ABA-responses were lower in agb1-2 than in WT, suggesting that those genes are possible downstream genes of AGB1 and that their changed expression plays an important role in determining phenotypic and physiologic traits in agb1-2. Taken together, these findings indicate that AGB1 positively regulates salt tolerance in Arabidopsis through its modulation of genes transcription related to proline biosynthesis, oxidative stress, ion homeostasis, stress- and ABA-responses.
学科主题	Agriculture, Multidisciplinary
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000349516100015
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4789]
专题	作物科学研究所_分子生物学系
作者单位	1.Northwest A&F Univ, Coll Agron, State Key Lab Arid Reg Crop Advers Biol, Yangling 712100, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Natl Key Lab Biol & Genet Improvement Triticeae C, Beijing 100081, Peoples R China 3.Beijing Hybrid Wheat Engn & Technol Res Ctr, Beijing 100097, Peoples R China
推荐引用方式 GB/T 7714	Ma Ya-nan,Chen Ming,Xu Dong-bei,et al. G-protein beta subunit AGB1 positively regulates salt stress tolerance in Arabidopsis[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2015,14(2):314-325.
APA	Ma Ya-nan.,Chen Ming.,Xu Dong-bei.,Fang Guang-ning.,Wang Er-hui.,...&Ma You-zhi.(2015).G-protein beta subunit AGB1 positively regulates salt stress tolerance in Arabidopsis.JOURNAL OF INTEGRATIVE AGRICULTURE,14(2),314-325.
MLA	Ma Ya-nan,et al."G-protein beta subunit AGB1 positively regulates salt stress tolerance in Arabidopsis".JOURNAL OF INTEGRATIVE AGRICULTURE 14.2(2015):314-325.