Co-expression of G2-EPSPS and glyphosate acetyltransferase GAT genes conferring high tolerance to glyphosate in soybean

doi:10.3389/fpls.2015.00847

	Co-expression of G2-EPSPS and glyphosate acetyltransferase GAT genes conferring high tolerance to glyphosate in soybean
	Guo, Bingfu 1,2; Guo, Yong 1; Hong, Huilong 1,2; Jin, Longguo 1; Zhang, Lijuan 1; Chang, Ru-Zhen 1; Lu, Wei 3; Lin, Min 3; Qiu, Li-Juan 1
刊名	FRONTIERS IN PLANT SCIENCE
	2015
卷号	6 页码:-
关键词	transgenic soybean glyphosate tolerance G2-EPSPS GAT chlorophyll content shikimic acid
ISSN号	1664-462X
DOI	10.3389/fpls.2015.00847
通讯作者	Guo, Bingfu
英文摘要	Glyphosate is a widely used non-selective herbicide with broad spectrum of weed control around the world. At present, most of the commercial glyphosate tolerant soybeans utilize glyphosate tolerant gene CP4-EPSPS or glyphosate acetyltransferase gene GAT separately. In this study, both glyphosate tolerant gene G2-EPSPS and glyphosate degraded gene GAT were co-transferred into soybean and transgenic plants showed high tolerance to glyphosate. Molecular analysis including PCR, Sothern blot, qRT-PCR, and Western blot revealed that target genes have been integrated into genome and expressed effectively at both mRNA and protein levels. Furthermore, the glyphosate tolerance analysis showed that no typical symptom was observed when compared with a glyphosate tolerant line HJ06-698 derived from GR1 transgenic soybean even at fourfold labeled rate of Roundup. Chlorophyll and shikimic acid content analysis of transgenic plant also revealed that these two indexes were not significantly altered after glyphosate application. These results indicated that co-expression of G2-EPSPS and GAT conferred high tolerance to the herbicide glyphosate in soybean. Therefore, combination of tolerant and degraded genes provides a new strategy for developing glyphosate tolerant transgenic crops.
学科主题	Plant Sciences
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000364241500001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4648]
专题	作物科学研究所_分子生物学系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100193, Peoples R China 2.Northeast Agr Univ, Coll Agr, Harbin, Peoples R China 3.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Guo, Bingfu,Guo, Yong,Hong, Huilong,et al. Co-expression of G2-EPSPS and glyphosate acetyltransferase GAT genes conferring high tolerance to glyphosate in soybean[J]. FRONTIERS IN PLANT SCIENCE,2015,6:-.
APA	Guo, Bingfu.,Guo, Yong.,Hong, Huilong.,Jin, Longguo.,Zhang, Lijuan.,...&Qiu, Li-Juan.(2015).Co-expression of G2-EPSPS and glyphosate acetyltransferase GAT genes conferring high tolerance to glyphosate in soybean.FRONTIERS IN PLANT SCIENCE,6,-.
MLA	Guo, Bingfu,et al."Co-expression of G2-EPSPS and glyphosate acetyltransferase GAT genes conferring high tolerance to glyphosate in soybean".FRONTIERS IN PLANT SCIENCE 6(2015):-.