Effect of La3+ on structure and electrochemical reaction of microperoxidase-11 in imitated physiological solution

	Effect of La3+ on structure and electrochemical reaction of microperoxidase-11 in imitated physiological solution
	Huang XH ; Guo SF ; Zhou Q ; Lu TH ; Ding XL
刊名	journal of electroanalytical chemistry
	2007
卷号	600 期号:2 页码:227-235
关键词	DIMETHYL-SULFOXIDE SOLUTION SELF-ASSEMBLED MONOLAYERS HEART CYTOCHROME-C HORSERADISH-PEROXIDASE ELECTRON-TRANSFER ELECTROCATALYTIC REDUCTION GOLD ELECTRODE GLASSY-CARBON CONFORMATIONAL-CHANGES NICKEL(II) PORPHYRINS
ISSN号	0022-0728
通讯作者	lu th
中文摘要	in this paper, the interaction mechanism between la3+ and microperoxidase-11 (mp-11) in the imitated physiological solution was investigated with the electrochemical and spectroscopic methods. it was found that when the molar ratio of la3+, and mp-11 is low, such as 2, la3+ can coordinate with oxygen in the propionic acid group of the heme group in the mp-11 molecule, forming the la-mp-11 complexes and leading to the increase in the non-planarity of the porphyrin cycle in the heme group and then the increase in the extent of exposure of the electrochemically active center, fe(i i i) in the porphyrin cycle of the heme group. the increase in the extent of exposure of the electrochemically active center, fe(iii) in the porphyrin cycle of the heme group would increase the reversibility of the electrochemical reaction of the la-mp-11 complexes and its electrocatalytic activity for the reduction of h2o2. the results of the chromatographic analysis demonstrated that the average molar ratio of la3+ and mp-11 in the la-mp-11 complexes is 1.62.when the molar ratio of la3+ and mp-11 is high, such as 3, la3+ would shear some amino acid residues of the peptide of mp-11. therefore, many la3+ ions can bind to the oxygen- and/or nitrogen-containing groups in the sheared amino acid residues except coordinating with the sheared and non-sheared mp-11 molecules.
收录类别	SCI
语种	英语
WOS记录号	WOS:000244510500001
公开日期	2010-07-13
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/14567]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Huang XH,Guo SF,Zhou Q,et al. Effect of La3+ on structure and electrochemical reaction of microperoxidase-11 in imitated physiological solution[J]. journal of electroanalytical chemistry,2007,600(2):227-235.
APA	Huang XH,Guo SF,Zhou Q,Lu TH,&Ding XL.(2007).Effect of La3+ on structure and electrochemical reaction of microperoxidase-11 in imitated physiological solution.journal of electroanalytical chemistry,600(2),227-235.
MLA	Huang XH,et al."Effect of La3+ on structure and electrochemical reaction of microperoxidase-11 in imitated physiological solution".journal of electroanalytical chemistry 600.2(2007):227-235.