Overexpression of TaNHX2 enhances salt tolerance of 'composite' and whole transgenic soybean plants

doi:10.1007/s11240-011-0005-9

	Overexpression of TaNHX2 enhances salt tolerance of 'composite' and whole transgenic soybean plants
	Cao, Dong 1,2,3; Hou, Wensheng 2; Liu, Wei 2; Yao, Weiwei 2; Wu, Cunxiang 2; Liu, Xiaobing 1; Han, Tianfu 2
刊名	PLANT CELL TISSUE AND ORGAN CULTURE
	2011
卷号	107 期号:3 页码:541-552
关键词	TaNHX2 Soybean Salt tolerance Transformation Agrobacterium rhizogenes Agrobacterium tumefaciens
ISSN号	0167-6857
DOI	10.1007/s11240-011-0005-9
通讯作者	Cao, Dong
英文摘要	Salinity is a major factor resulting in extensive loss of agricultural production. Genetic transformation has become a powerful tool for studying gene function and for improving crop salt tolerance. In this study, a TaNHX2 gene was transformed into a plant cloning vector under the control of cauliflower mosaic virus 35S promoter, and then introduced into Agrobacterium rhizogenes strain K599. Explants of soybean were transformed with A. rhizogenes and 'composite' plants consisting of wild-type shoots and transgenic hairy roots overexpressing TaNHX2 were produced. When exposed to salt stress, 'composite' plants displayed high salinity tolerance at 171 mM NaCl in vermiculite and in solid medium supplemented with up to 200 mM NaCl, whereas control plants displayed chlorosis and died within 15 days under above treatment conditions. We subsequently obtained soybean plants overexpressing TaNHX2 through A. tumefaciens-mediated transformation and studied four homozygous lines of TaNHX2. Transgenic lines displayed an enhanced salt tolerance in plant biomass and flower number per plant, compared with wild type plants grown on sand culture containing 150 mM NaCl. Furthermore, transgenic plants of line C12-11 showed longer survival, less growth inhibition and greater number of flowers than wild type plants. Taken together, these results indicated that TaNHX2 gene could enhance salt tolerance of soybean, and A. rhizogenes-mediated transformation system could be used as a complementary tool of A. tumerfaciens-mediated transformation to rapidly investigate candidate gene function in soybean.
学科主题	Biotechnology & Applied Microbiology ; Plant Sciences ; BIOTECHNOLOGY & APPLIED MICROBIOLOGY ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000300371300017
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5244]
专题	作物科学研究所_遗传育种系
作者单位	1.Chinese Acad Sci, NE Inst Geog & Agroecol, Key Lab Mollisols Agroecol, Harbin 150081, Heilongjiang, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Cao, Dong,Hou, Wensheng,Liu, Wei,et al. Overexpression of TaNHX2 enhances salt tolerance of 'composite' and whole transgenic soybean plants[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2011,107(3):541-552.
APA	Cao, Dong.,Hou, Wensheng.,Liu, Wei.,Yao, Weiwei.,Wu, Cunxiang.,...&Han, Tianfu.(2011).Overexpression of TaNHX2 enhances salt tolerance of 'composite' and whole transgenic soybean plants.PLANT CELL TISSUE AND ORGAN CULTURE,107(3),541-552.
MLA	Cao, Dong,et al."Overexpression of TaNHX2 enhances salt tolerance of 'composite' and whole transgenic soybean plants".PLANT CELL TISSUE AND ORGAN CULTURE 107.3(2011):541-552.