Chlorophyll Deficiency in the Maize elongated mesocotyl2 Mutant Is Caused by a Defective Heme Oxygenase and Delaying Grana Stacking

doi:10.1371/journal.pone.0080107

	Chlorophyll Deficiency in the Maize elongated mesocotyl2 Mutant Is Caused by a Defective Heme Oxygenase and Delaying Grana Stacking
	Shi, Dianyi 1,2; Zheng, Xu 3; Li, Liang 1; Lin, Wanhuang 4; Xie, Wenjun 1; Yang, Jianping 3; Chen, Shaojiang 1; Jin, Weiwei 1
刊名	PLOS ONE
	2013
卷号	8 期号:11 页码:-
ISSN号	1932-6203
DOI	10.1371/journal.pone.0080107
通讯作者	Shi, Dianyi
英文摘要	Background: Etiolated seedlings initiate grana stacking and chlorophyll biosynthesis in parallel with the first exposure to light, during which phytochromes play an important role. Functional phytochromes are biosynthesized separately for two components. One phytochrome is biosynthesized for apoprotein and the other is biosynthesized for the chromophore that includes heme oxygenase (HO).Methodology/Principal Finding: We isolated a ho1 homolog by map-based cloning of a maize elongated mesocotyl2 (elm2) mutant. cDNA sequencing of the ho1 homolog in elm2 revealed a 31 bp deletion. De-etiolation responses to red and far-red light were disrupted in elm2 seedlings, with a pronounced elongation of the mesocotyl. The endogenous HO activity in the elm2 mutant decreased remarkably. Transgenic complementation further confirmed the dysfunction in the maize ho1 gene. Moreover, non-appressed thylakoids were specifically stacked at the seedling stage in the elm2 mutant.Conclusion: The 31 bp deletion in the ho1 gene resulted in a decrease in endogenous HO activity and disrupted the de-etiolation responses to red and far-red light. The specific stacking of non-appressed thylakoids suggested that the chlorophyll biosynthesis regulated by HO1 is achieved by coordinating the heme level with the regulation of grana stacking.
学科主题	Multidisciplinary Sciences ; MULTIDISCIPLINARY SCIENCES
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000327221600180
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4985]
专题	作物科学研究所_遗传育种系
作者单位	1.China Agr Univ, Minist Agr, Key Lab Crop Genet Improvement & Genome, Natl Maize Improvement Ctr China, Beijing 100094, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Natl Key Lab Plant Mol Genet, Shanghai, Peoples R China 3.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100193, Peoples R China 4.Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha, Hunan, Peoples R China
推荐引用方式 GB/T 7714	Shi, Dianyi,Zheng, Xu,Li, Liang,et al. Chlorophyll Deficiency in the Maize elongated mesocotyl2 Mutant Is Caused by a Defective Heme Oxygenase and Delaying Grana Stacking[J]. PLOS ONE,2013,8(11):-.
APA	Shi, Dianyi.,Zheng, Xu.,Li, Liang.,Lin, Wanhuang.,Xie, Wenjun.,...&Jin, Weiwei.(2013).Chlorophyll Deficiency in the Maize elongated mesocotyl2 Mutant Is Caused by a Defective Heme Oxygenase and Delaying Grana Stacking.PLOS ONE,8(11),-.
MLA	Shi, Dianyi,et al."Chlorophyll Deficiency in the Maize elongated mesocotyl2 Mutant Is Caused by a Defective Heme Oxygenase and Delaying Grana Stacking".PLOS ONE 8.11(2013):-.