Ectopic expression of a phytochrome B gene from Chinese cabbage (Brassica rapa L. ssp pekinensis) in Arabidopsis thaliana promotes seedling de-etiolation, dwarfing in mature plants, and delayed flowering

doi:10.1007/s11103-015-0302-5

	Ectopic expression of a phytochrome B gene from Chinese cabbage (Brassica rapa L. ssp pekinensis) in Arabidopsis thaliana promotes seedling de-etiolation, dwarfing in mature plants, and delayed flowering
	Song, Mei-Fang 1,2,3; Zhang, Shu 2; Hou, Pei 3; Shang, Hong-Zhong 1; Gu, Hai-Ke 1; Li, Jing-Juan 2; Xiao, Yang 4; Guo, Lin 3; Su, Liang 3; Gao, Jian-Wei 2
刊名	PLANT MOLECULAR BIOLOGY
	2015
卷号	87 期号:6 页码:633-643
关键词	Arabidopsis thaliana Chinese cabbage Ectopic expression De-etiolation Flowering time Phytochrome B
ISSN号	0167-4412
DOI	10.1007/s11103-015-0302-5
通讯作者	Song, Mei-Fang
英文摘要	Phytochrome B (phyB) is an essential red light receptor that predominantly mediates seedling de-etiolation, shade-avoidance response, and flowering time. In this study, we isolate a full-length cDNA of PHYB, designated BrPHYB, from Chinese cabbage (Brassica rapa L. ssp. pekinensis), and we find that BrphyB protein has high amino acid sequence similarity and the closest evolutionary relationship to Arabidopsis thaliana phyB (i.e., AtphyB). Quantitative reverse transcription (RT)-PCR results indicate that the BrPHYB gene is ubiquitously expressed in different tissues under all light conditions. Constitutive expression of the BrPHYB gene in A. thaliana significantly enhances seedling de-etiolation under red- and white-light conditions, and causes dwarf stature in mature plants. Unexpectedly, overexpression of BrPHYB in transgenic A. thaliana resulted in reduced expression of gibberellins biosynthesis genes and delayed flowering under short-day conditions, whereas AtPHYB overexpression caused enhanced expression of FLOWERING LOCUS T and earlier flowering. Our results suggest that BrphyB might play an important role in regulating the development of Chinese cabbage. BrphyB and AtphyB have conserved functions during de-etiolation and vegetative plant growth and divergent functions in the regulation of flowering time.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000352086700008
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4735]
专题	作物科学研究所_遗传育种系
作者单位	1.Beijing Radiat Ctr, Beijing 100875, Peoples R China 2.Shandong Acad Agr Sci, Inst Vegetables & Flowers, Shandong Key Lab Greenhouse Vegetable Biol, Jinan 250100, Peoples R China 3.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China 4.Chinese Acad Agr Sci, Grad Sch, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Song, Mei-Fang,Zhang, Shu,Hou, Pei,et al. Ectopic expression of a phytochrome B gene from Chinese cabbage (Brassica rapa L. ssp pekinensis) in Arabidopsis thaliana promotes seedling de-etiolation, dwarfing in mature plants, and delayed flowering[J]. PLANT MOLECULAR BIOLOGY,2015,87(6):633-643.
APA	Song, Mei-Fang.,Zhang, Shu.,Hou, Pei.,Shang, Hong-Zhong.,Gu, Hai-Ke.,...&Yang, Jian-Ping.(2015).Ectopic expression of a phytochrome B gene from Chinese cabbage (Brassica rapa L. ssp pekinensis) in Arabidopsis thaliana promotes seedling de-etiolation, dwarfing in mature plants, and delayed flowering.PLANT MOLECULAR BIOLOGY,87(6),633-643.
MLA	Song, Mei-Fang,et al."Ectopic expression of a phytochrome B gene from Chinese cabbage (Brassica rapa L. ssp pekinensis) in Arabidopsis thaliana promotes seedling de-etiolation, dwarfing in mature plants, and delayed flowering".PLANT MOLECULAR BIOLOGY 87.6(2015):633-643.