Cloning and characterization of a maize SnRK2 protein kinase gene confers enhanced salt tolerance in transgenic Arabidopsis

doi:10.1007/s00299-011-1077-z

	Cloning and characterization of a maize SnRK2 protein kinase gene confers enhanced salt tolerance in transgenic Arabidopsis
	Ying, Sheng 1,2; Zhang, Deng-Feng 1; Li, Hui-Yong 1,3; Liu, Ying-Hui 1,4; Shi, Yun-Su 1; Song, Yan-Chun 1; Wang, Tian-Yu 1; Li, Yu 1
刊名	PLANT CELL REPORTS
	2011
卷号	30 期号:9 页码:1683-1699
关键词	Maize Salt tolerance SnRK2 ZmSAPK8
ISSN号	0721-7714
DOI	10.1007/s00299-011-1077-z
通讯作者	Ying, Sheng
英文摘要	SnRK2 (sucrose non-fermenting 1-related protein kinases 2) represents a unique family of protein kinase in regulating signaling transduction in plants. Although the regulatory mechanisms of SnRK2 have been well demonstrated in Arabidopsis thaliana, their functions in maize are still unknown. In our study, we cloned an SnRK2 gene from maize, ZmSAPK8, which encoded a putative homolog of the rice SAPK8 protein. ZmSAPK8 had two copies in the maize genome and harbored eight introns in its coding region. We demonstrated that ZmSAPK8 expressed differentially in various organs of maize plants and was up-regulated by high-salinity and drought treatment. A green fluorescent protein (GFP)-tagged ZmSAPK8 showed subcellular localization in the cell membrane, cytoplasm and nucleus. In vitro kinase assays indicated that ZmSAPK8 preferred Mn(2+) to Mg(2+) as cofactor for phosphorylation, and Ser-182 and Thr-183 in activation loop was important for its activity. Heterologous overexpression of ZmSAPK8 in Arabidopsis could significantly strengthen tolerance to salt stress. Under salt treatment, ZmSAPK8-overexpressed transgenic plants exhibited higher germination rate and proline content, low electrolyte leakage and higher survival rate than wild type. Further analysis indicated that transgenic plants showed increased transcription of the stress-related genes, RD29A, RD29B, RAB18, ABI1, DREB2A and P5CS1, under high-salinity conditions. The results demonstrated that ZmSAPK8 was involved in diverse stress signal transduction. Moreover, no obvious adverse effects on growth and development in the ZmSAPK8-overexpressed transgenic plants implied that ZmSAPK8 was potentially useful in transgenic breeding to improve salt tolerance in crops.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000293794500009
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5272]
专题	作物科学研究所_种质资源保存与研究中心
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China 2.China Agr Univ, Coll Biol Sci, Beijing 100193, Peoples R China 3.Henan Acad Agr Sci, Cereal Crops Inst, Zhengzhou 450002, Peoples R China 4.Heibei N Univ, Zhangjiakou 075000, Peoples R China
推荐引用方式 GB/T 7714	Ying, Sheng,Zhang, Deng-Feng,Li, Hui-Yong,et al. Cloning and characterization of a maize SnRK2 protein kinase gene confers enhanced salt tolerance in transgenic Arabidopsis[J]. PLANT CELL REPORTS,2011,30(9):1683-1699.
APA	Ying, Sheng.,Zhang, Deng-Feng.,Li, Hui-Yong.,Liu, Ying-Hui.,Shi, Yun-Su.,...&Li, Yu.(2011).Cloning and characterization of a maize SnRK2 protein kinase gene confers enhanced salt tolerance in transgenic Arabidopsis.PLANT CELL REPORTS,30(9),1683-1699.
MLA	Ying, Sheng,et al."Cloning and characterization of a maize SnRK2 protein kinase gene confers enhanced salt tolerance in transgenic Arabidopsis".PLANT CELL REPORTS 30.9(2011):1683-1699.