A Determination of Potential alpha-Glucosidase Inhibitors from Azuki Beans (Vigna angularis)

doi:10.3390/ijms12106445

	A Determination of Potential alpha-Glucosidase Inhibitors from Azuki Beans (Vigna angularis)
	Yao, Yang; Cheng, Xuzhen; Wang, Lixia; Wang, Suhua; Ren, Guixing
刊名	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
	2011
卷号	12 期号:10 页码:6445-6451
关键词	azuki beans alpha-glucosidase inhibitory vitexin isovitexin
ISSN号	1422-0067
DOI	10.3390/ijms12106445
通讯作者	Yao, Yang
英文摘要	A 70% ethanol extract from azuki beans (Vigna angularis) was extracted further with CH2Cl2, EtOAc and n-BuOH to afford four fractions: CH2Cl2-soluble, EtOAc-soluble, n-BuOH-soluble and residual extract fractions. The EtOAc-soluble fractions showed the highest alpha-glucosidase inhibitory activity. Two pure flavonoid compounds, vitexin and isovitexin, were isolated (using the enzyme assay-guide fractionation method) from the EtOAc-soluble fractions. We further evaluated the interaction between the flavonoid compounds and alpha-glucosidase by fluorescence spectroscopy. Vitexin and isovitexin showed high inhibitory activities, with IC50 values of 0.4 mg.mL(-1) and 4.8 mg.mL(-1), respectively. This is the first study of the active compositions of azuki beans against alpha-glucosidase.
学科主题	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary ; CHEMISTRY, MULTIDISCIPLINARY
语种	英语
出版者	MDPI AG
WOS记录号	WOS:000296569600007
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5266]
专题	作物科学研究所_种质资源保存与研究中心
作者单位	Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Yao, Yang,Cheng, Xuzhen,Wang, Lixia,et al. A Determination of Potential alpha-Glucosidase Inhibitors from Azuki Beans (Vigna angularis)[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2011,12(10):6445-6451.
APA	Yao, Yang,Cheng, Xuzhen,Wang, Lixia,Wang, Suhua,&Ren, Guixing.(2011).A Determination of Potential alpha-Glucosidase Inhibitors from Azuki Beans (Vigna angularis).INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,12(10),6445-6451.
MLA	Yao, Yang,et al."A Determination of Potential alpha-Glucosidase Inhibitors from Azuki Beans (Vigna angularis)".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 12.10(2011):6445-6451.