High-throughput novel microsatellite marker of faba bean via next generation sequencing

doi:10.1186/1471-2164-13-602

	High-throughput novel microsatellite marker of faba bean via next generation sequencing
	Yang, Tao 1; Bao, Shi-Ying 2; Ford, Rebecca 3; Jia, Teng-Jiao 1; Guan, Jian-Ping 1; He, Yu-Hua 2; Sun, Xue-Lian 1; Jiang, Jun-Ye 1; Hao, Jun-Jie 4; Zhang, Xiao-Yan 4
刊名	BMC GENOMICS
	2012
卷号	13 页码:-
关键词	Microsatellite markers Next generation sequencing Marker development Vicia faba L
ISSN号	1471-2164
DOI	10.1186/1471-2164-13-602
通讯作者	Yang, Tao
英文摘要	Background: Faba bean (Vicia faba L.) is an important food legume crop, grown for human consumption globally including in China, Turkey, Egypt and Ethiopia. Although genetic gain has been made through conventional selection and breeding efforts, this could be substantially improved through the application of molecular methods. For this, a set of reliable molecular markers representative of the entire genome is required.Results: A library with 125,559 putative SSR sequences was constructed and characterized for repeat type and length from a mixed genome of 247 spring and winter sown faba bean genotypes using 454 sequencing. A suit of 28,503 primer pair sequences were designed and 150 were randomly selected for validation. Of these, 94 produced reproducible amplicons that were polymorphic among 32 faba bean genotypes selected from diverse geographical locations. The number of alleles per locus ranged from 2 to 8, the expected heterozygocities ranged from 0.0000 to 1.0000, and the observed heterozygosities ranged from 0.0908 to 0.8410. The validation by UPGMA cluster analysis of 32 genotypes based on Nei's genetic distance, showed high quality and effectiveness of those novel SSR markers developed via next generation sequencing technology.Conclusions: Large scale SSR marker development was successfully achieved using next generation sequencing of the V. faba genome. These novel markers are valuable for constructing genetic linkage maps, future QTL mapping, and marker-assisted trait selection in faba bean breeding efforts.
学科主题	Biotechnology & Applied Microbiology ; Genetics & Heredity ; BIOTECHNOLOGY & APPLIED MICROBIOLOGY ; GENETICS & HEREDITY
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000314647400002
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5117]
专题	作物科学研究所_种质资源保存与研究中心
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 2.Yunnan Acad Agr Sci, Inst Grain Crops, Kunming 650205, Peoples R China 3.Univ Melbourne, Melbourne Sch Land & Environm, Dept Agr & Food Syst, Melbourne, Vic 3010, Australia 4.Qingdao Acad Agr Sci, Qingdao 266100, Peoples R China
推荐引用方式 GB/T 7714	Yang, Tao,Bao, Shi-Ying,Ford, Rebecca,et al. High-throughput novel microsatellite marker of faba bean via next generation sequencing[J]. BMC GENOMICS,2012,13:-.
APA	Yang, Tao.,Bao, Shi-Ying.,Ford, Rebecca.,Jia, Teng-Jiao.,Guan, Jian-Ping.,...&Zong, Xu-Xiao.(2012).High-throughput novel microsatellite marker of faba bean via next generation sequencing.BMC GENOMICS,13,-.
MLA	Yang, Tao,et al."High-throughput novel microsatellite marker of faba bean via next generation sequencing".BMC GENOMICS 13(2012):-.