Efficient Induction of Wheat-Agropyron cristatum 6P Translocation Lines and GISH Detection

doi:10.1371/journal.pone.0069501

	Efficient Induction of Wheat-Agropyron cristatum 6P Translocation Lines and GISH Detection
	Song, Liqiang; Jiang, Lili; Han, Haiming; Gao, Ainong; Yang, Xinming; Li, Lihui; Liu, Weihua
刊名	PLOS ONE
	2013
卷号	8 期号:7 页码:-
ISSN号	1932-6203
DOI	10.1371/journal.pone.0069501
通讯作者	Song, Liqiang
英文摘要	The narrow genetic background restricts wheat yield and quality improvement. The wild relatives of wheat are the huge gene pools for wheat improvement and can broaden its genetic basis. Production of wheat-alien translocation lines can transfer alien genes to wheat. So it is important to develop an efficient method to induce wheat-alien chromosome translocation. Agropyron cristatum (P genome) carries many potential genes beneficial to disease resistance, stress tolerance and high yield. Chromosome 6P possesses the desirable genes exhibiting good agronomic traits, such as high grain number per spike, powdery mildew resistance and stress tolerance. In this study, the wheat-A. cristatum disomic addition was used as bridge material to produce wheat-A. cristatum translocation lines induced by Co-60-gamma irradiation. The results of genomic in situ hybridization showed that 216 plants contained alien chromosome translocation among 571 self-pollinated progenies. The frequency of translocation was 37.83%, much higher than previous reports. Moreover, various alien translocation types were identified. The analysis of M-2 showed that 62.5% of intergeneric translocation lines grew normally without losing the translocated chromosomes. The paper reported a high efficient technical method for inducing alien translocation between wheat and Agropyron cristatum. Additionally, these translocation lines will be valuable for not only basic research on genetic balance, interaction and expression of different chromosome segments of wheat and alien species, but also wheat breeding programs to utilize superior agronomic traits and good compensation effect from alien chromosomes.
学科主题	Multidisciplinary Sciences ; MULTIDISCIPLINARY SCIENCES
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000321341000192
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5027]
专题	作物科学研究所_种质资源保存与研究中心
作者单位	Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Song, Liqiang,Jiang, Lili,Han, Haiming,et al. Efficient Induction of Wheat-Agropyron cristatum 6P Translocation Lines and GISH Detection[J]. PLOS ONE,2013,8(7):-.
APA	Song, Liqiang.,Jiang, Lili.,Han, Haiming.,Gao, Ainong.,Yang, Xinming.,...&Liu, Weihua.(2013).Efficient Induction of Wheat-Agropyron cristatum 6P Translocation Lines and GISH Detection.PLOS ONE,8(7),-.
MLA	Song, Liqiang,et al."Efficient Induction of Wheat-Agropyron cristatum 6P Translocation Lines and GISH Detection".PLOS ONE 8.7(2013):-.