Optimization of droplet-vitrification protocol for carnation genotypes and ultrastructural studies on shoot tips during cryopreservation

doi:10.1007/s11738-014-1685-7

	Optimization of droplet-vitrification protocol for carnation genotypes and ultrastructural studies on shoot tips during cryopreservation
	Zhang, Jin-Mei 1; Zhang, Xiao-Ning 1,2,3; Lu, Xin-Xiong ; Xin, Xia 1; Yin, Guang-Kun 1; He, Juan-Juan 1; Xu, You-Ming 2; Chen, Xiao-Ling 1
刊名	ACTA PHYSIOLOGIAE PLANTARUM
	2014
卷号	36 期号:12 页码:3189-3198
关键词	Carnation Dianthus caryophyllus L. Droplet-vitrification In vitro Shoot tips Ultrastructural investigation
ISSN号	0137-5881
DOI	10.1007/s11738-014-1685-7
通讯作者	Zhang, Jin-Mei
英文摘要	This study was carried out to optimize a modified droplet-vitrification procedure for the cryopreservation of shoot tips from different carnation genotypes. The best procedure was developed by applying orthogonal tests to the experimental data and by further investigation of the effects on the regrowth percentage. It consisted in preculturing shoot tips in liquid Murashige and Skoog (MS) medium with 0.3 M sucrose for 2 days, pretreating them in liquid MS medium with 5 % Dimethyl sulfoxide +5 % glycerol + 0.3 M sucrose for 10 min, osmoprotecting in Loading solution for 20 min at 25 A degrees C, cryoprotecting with Plant vitrification solution No.2 (PVS2) for 60 min at 0 A degrees C, transferring in drops of fresh PVS2 over aluminum strips and finally storing them in Liquid nitrogen. With the application of the optimized protocol, four carnation cultivars ('Master', 'Calibra', 'Lamour' and 'Ofcar') achieved regrowth percentage after cryopreservation ranging from 41 to 73 %. Ultrastructural observations investigated by using transmission electron microscopy showed that the cells encountered the stress during cryopreservation and the main damages occurred during the dehydration step. For surviving cells, the most of the damaged cells could be repaired after recovery growth. This modified protocol will aid in the long-term conservation of carnation germplasm and the ultrastructural studies will benefit for understanding the damage and recovery of the cells during cryopreservation.
学科主题	Plant Sciences
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000344338300009
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4800]
专题	作物科学研究所_种质资源保存与研究中心
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Genebank, Beijing 100081, Peoples R China 2.Huazhong Agr Univ, Coll Hort & Forestry Sci, Wuhan 430070, Peoples R China 3.JiangXi Acad Agr Sci, Inst Rice Res, Nanchang 330200, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Jin-Mei,Zhang, Xiao-Ning,Lu, Xin-Xiong,et al. Optimization of droplet-vitrification protocol for carnation genotypes and ultrastructural studies on shoot tips during cryopreservation[J]. ACTA PHYSIOLOGIAE PLANTARUM,2014,36(12):3189-3198.
APA	Zhang, Jin-Mei.,Zhang, Xiao-Ning.,Lu, Xin-Xiong.,Xin, Xia.,Yin, Guang-Kun.,...&Chen, Xiao-Ling.(2014).Optimization of droplet-vitrification protocol for carnation genotypes and ultrastructural studies on shoot tips during cryopreservation.ACTA PHYSIOLOGIAE PLANTARUM,36(12),3189-3198.
MLA	Zhang, Jin-Mei,et al."Optimization of droplet-vitrification protocol for carnation genotypes and ultrastructural studies on shoot tips during cryopreservation".ACTA PHYSIOLOGIAE PLANTARUM 36.12(2014):3189-3198.