Differentially Expressed Genes in Resistant and Susceptible Common Bean (Phaseolus vulgaris L.) Genotypes in Response to Fusarium oxysporum f. sp phaseoli

doi:10.1371/journal.pone.0127698

	Differentially Expressed Genes in Resistant and Susceptible Common Bean (Phaseolus vulgaris L.) Genotypes in Response to Fusarium oxysporum f. sp phaseoli
	Xue, Renfeng 1,2; Wu, Jing 2; Zhu, Zhendong 2; Wang, Lanfen 2; Wang, Xiaoming 2; Wang, Shumin 2; Blair, Matthew W.3
刊名	PLOS ONE
	2015
卷号	10 期号:6 页码:-
ISSN号	1932-6203
DOI	10.1371/journal.pone.0127698
通讯作者	Xue, Renfeng
英文摘要	Fusarium wilt of common bean (Phaseolus vulgaris L.), caused by Fusarium oxysporum Schlechtend.:Fr. f.sp. phaseoli (Fop), is one of the most important diseases of common beans worldwide. Few natural sources of resistance to Fop exist and provide only moderate or partial levels of protection. Despite the economic importance of the disease across multiple crops, only a few of Fop induced genes have been analyzed in legumes. Therefore, our goal was to identify transcriptionally regulated genes during an incompatible interaction between common bean and the Fop pathogen using the cDNA amplified fragment length polymorphism (cDNA-AFLP) technique. We generated a total of 8,730 transcript-derived fragments (TDFs) with 768 primer pairs based on the comparison of a moderately resistant and a susceptible genotype. In total, 423 TDFs (4.9%) displayed altered expression patterns after inoculation with Fop inoculum. We obtained full amplicon sequences for 122 selected TDFs, of which 98 were identified as annotated known genes in different functional categories based on their putative functions, 10 were predicted but non-annotated genes and 14 were not homologous to any known genes. The 98 TDFs encoding genes of known putative function were classified as related to metabolism(22), signal transduction (21), protein synthesis and processing (20), development and cytoskeletal organization (12), transport of proteins (7), gene expression and RNA metabolism (4), redox reactions (4), defense and stress responses (3), energy metabolism (3), and hormone responses (2). Based on the analyses of homology, 19 TDFs from different functional categories were chosen for expression analysis using quantitative RT-PCR. The genes found to be important here were implicated at various steps of pathogen infection and will allow a better understanding of the mechanisms of defense and resistance to Fop and similar pathogens. The differential response genes discovered here could also be used asmolecular markers in association mapping or QTL analysis.
学科主题	Multidisciplinary Sciences
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000356630900073
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4703]
专题	作物科学研究所_种质资源保存与研究中心
作者单位	1.Liaoning Acad Agr Sci, Crop Res Inst, Shenyang, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Fac Crop Gene Resources & Genet Improvem, Beijing 100193, Peoples R China 3.Tennessee State Univ, Dept Agr & Environm Sci, Nashville, TN 37203 USA
推荐引用方式 GB/T 7714	Xue, Renfeng,Wu, Jing,Zhu, Zhendong,et al. Differentially Expressed Genes in Resistant and Susceptible Common Bean (Phaseolus vulgaris L.) Genotypes in Response to Fusarium oxysporum f. sp phaseoli[J]. PLOS ONE,2015,10(6):-.
APA	Xue, Renfeng.,Wu, Jing.,Zhu, Zhendong.,Wang, Lanfen.,Wang, Xiaoming.,...&Blair, Matthew W..(2015).Differentially Expressed Genes in Resistant and Susceptible Common Bean (Phaseolus vulgaris L.) Genotypes in Response to Fusarium oxysporum f. sp phaseoli.PLOS ONE,10(6),-.
MLA	Xue, Renfeng,et al."Differentially Expressed Genes in Resistant and Susceptible Common Bean (Phaseolus vulgaris L.) Genotypes in Response to Fusarium oxysporum f. sp phaseoli".PLOS ONE 10.6(2015):-.