Cloning of the OAT gene and the correlation between its expression and drought tolerance in Phaseolus vulgaris L.

doi:10.1016/S2095-3119(15)61283-7

	Cloning of the OAT gene and the correlation between its expression and drought tolerance in Phaseolus vulgaris L.
	Chen Ji-bao 1; Cao Yuan-nan 1; Zhang Zhao-yuan 1; Wang Shu-min 2; Wu Jing 2; Wang Lan-fen 2
刊名	JOURNAL OF INTEGRATIVE AGRICULTURE
	2016
卷号	15 期号:5 页码:973-982
关键词	common bean ornithine aminotransferase gene proline drought tolerance
ISSN号	2095-3119
DOI	10.1016/S2095-3119(15)61283-7
通讯作者	Chen Ji-bao
英文摘要	Drought stress is a major abiotic stress of common bean (Phaseolus vulgaris L.) throughout the world. Increasing the proline accumulation contributes to enhance crop drought tolerance. A cDNA for delta-ornithine aminotransferase (delta-OAT), an enzyme involved in the biosynthesis of proline, was isolated from Phaseolus vulgaris (PvOAT). PvOAT exhibits 87.4 and 39.8% similarity of the deduced amino acid sequences with delta-OAT from Glycine max and Vigna aconitifolia, respectively. The transcriptional analysis revealed that PvOAT was strongly induced by drought stress. And the expression of PvOAT was higher in leaves than that in the root and stem of common bean by drought stress. Similar increase of the proline accumulation was observed in leaves and roots of common bean by drought stress. Furthermore, the proline content, the PvOAT expression and the PvOAT enzyme activity in cultivar F5575 was significantly (P<0.01) higher than that in cultivar F4851 under drought-stress conditions. Interestingly, it had been observed that, in the later stage of drought stress, the proline steadily maintained at the maximum level maybe result from the PvOAT enzyme activity increasing steadily. These results indicated that the expression of PvOAT and the accumulation of proline induced by drought stress treatment were related to the degree of common bean drought tolerance. So our results support the view that delta-OAT is associated with proline synthesis under drought stress conditions.
学科主题	Agriculture, Multidisciplinary
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000376144700005
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4594]
专题	作物科学研究所_种质资源保存与研究中心
作者单位	1.Nanyang Normal Univ, Sch Life Sci & Technol, Nanyang 473061, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Chen Ji-bao,Cao Yuan-nan,Zhang Zhao-yuan,et al. Cloning of the OAT gene and the correlation between its expression and drought tolerance in Phaseolus vulgaris L.[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2016,15(5):973-982.
APA	Chen Ji-bao,Cao Yuan-nan,Zhang Zhao-yuan,Wang Shu-min,Wu Jing,&Wang Lan-fen.(2016).Cloning of the OAT gene and the correlation between its expression and drought tolerance in Phaseolus vulgaris L..JOURNAL OF INTEGRATIVE AGRICULTURE,15(5),973-982.
MLA	Chen Ji-bao,et al."Cloning of the OAT gene and the correlation between its expression and drought tolerance in Phaseolus vulgaris L.".JOURNAL OF INTEGRATIVE AGRICULTURE 15.5(2016):973-982.