Joint-linkage mapping and GWAS reveal extensive genetic loci that regulate male inflorescence size in maize

doi:10.1111/pbi.12519

	Joint-linkage mapping and GWAS reveal extensive genetic loci that regulate male inflorescence size in maize
	Wu, Xun 1,5; Li, Yongxiang 1; Shi, Yunsu 1; Song, Yanchun 1; Zhang, Dengfeng 1; Li, Chunhui 1; Buckler, Edward S.2,3; Li, Yu 1; Zhang, Zhiwu 4,6; Wang, Tianyu 1
刊名	PLANT BIOTECHNOLOGY JOURNAL
	2016
卷号	14 期号:7 页码:1551-1562
关键词	maize male inflorescence size joint-linkage GWAS QTLs candidate gene
ISSN号	1467-7644
DOI	10.1111/pbi.12519
通讯作者	Wu, Xun
英文摘要	Both insufficient and excessive male inflorescence size leads to a reduction in maize yield. Knowledge of the genetic architecture of male inflorescence is essential to achieve the optimum inflorescence size for maize breeding. In this study, we used approximately eight thousand inbreds, including both linkage populations and association populations, to dissect the genetic architecture of male inflorescence. The linkage populations include 25 families developed in the U.S. and 11 families developed in China. Each family contains approximately 200 recombinant inbred lines (RILs). The association populations include approximately 1000 diverse lines from the U.S. and China. All inbreds were genotyped by either sequencing or microarray. Inflorescence size was measured as the tassel primary branch number (TBN) and tassel length (TL). A total of 125 quantitative trait loci (QTLs) were identified (63 for TBN, 62 for TL) through linkage analyses. In addition, 965 quantitative trait nucleotides (QTNs) were identified through genomewide study (GWAS) at a bootstrap posterior probability (BPP) above a 5% threshold. These QTLs/QTNs include 24 known genes that were cloned using mutants, for example Ramosa3 (ra3), Thick tassel dwarf1 (td1), tasselseed2 (ts2), liguleless2 (lg2), ramosa1 (ra1), barren stalk1 (ba1), branch silkless1 (bd1) and tasselseed6 (ts6). The newly identified genes encode a zinc transporter (e.g. GRMZM5G838098 and GRMZM2G047762), the adapt in terminal region protein (e.g. GRMZM5G885628), O-methyl-transferase (e.g. GRMZM2G147491), helix-loop-helix (HLH) DNA-binding proteins (e.g. GRMZM2G414252 and GRMZM2G042895) and an SBP-box protein (e.g. GRMZM2G058588). These results provide extensive genetic information to dissect the genetic architecture of inflorescence size for the improvement of maize yield.
学科主题	Biotechnology & Applied Microbiology ; Plant Sciences
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000378741200007
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4496]
专题	作物科学研究所_种质资源保存与研究中心
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Beijing, Peoples R China 2.Cornell Univ, Inst Genom Divers, Ithaca, NY 14850 USA 3.ARS, USDA, Ithaca, NY 14853 USA 4.Northeast Agr Univ, Dept Agron, Harbin, Heilongjiang, Peoples R China 5.Nanchong Acad Agr Sci, Nanchong, Sichuan, Peoples R China 6.Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA
推荐引用方式 GB/T 7714	Wu, Xun,Li, Yongxiang,Shi, Yunsu,et al. Joint-linkage mapping and GWAS reveal extensive genetic loci that regulate male inflorescence size in maize[J]. PLANT BIOTECHNOLOGY JOURNAL,2016,14(7):1551-1562.
APA	Wu, Xun.,Li, Yongxiang.,Shi, Yunsu.,Song, Yanchun.,Zhang, Dengfeng.,...&Wang, Tianyu.(2016).Joint-linkage mapping and GWAS reveal extensive genetic loci that regulate male inflorescence size in maize.PLANT BIOTECHNOLOGY JOURNAL,14(7),1551-1562.
MLA	Wu, Xun,et al."Joint-linkage mapping and GWAS reveal extensive genetic loci that regulate male inflorescence size in maize".PLANT BIOTECHNOLOGY JOURNAL 14.7(2016):1551-1562.