Improvement of Plant Regeneration from Immature Embryos of Wheat Infected by Agrobacterium tumefaciens

CORC > 中国农业科学院 > 作物科学研究所

	Improvement of Plant Regeneration from Immature Embryos of Wheat Infected by Agrobacterium tumefaciens
	TAO Li-li; YIN Gui-xiang; DU Li-pu; SHI Zheng-yuan; SHE Mao-yun; XU Hui-jun; YE Xing-guo
刊名	Agricultural Sciences in China
	2011
卷号	10 期号:3 页码:317-326
关键词	wheat immature embryos Agrobacterium tumefaciens transformation genotypes plant regeneration
ISSN号	1671-2927
英文摘要	Wheat,one of the most important food crops,has been extensively studied with respects to plant regeneration and transformation employing the immature embryos as recipient tissues.However,the transformed tissues often become severely necrotic after co-cultivation with Agrobacterium,which is one of the major obstacles in gene delivery.In this study,wheat varieties CB037,Kenong 199,Xinchun 9,Lunxuan 987,and Shi 4185 showed desirable culture potential or high infection ability in Agrobacterium-mediated transformation.Similarly,optimal regeneration conditions were determined by testing their ability to inhibit the cell necrosis and cell death phenotype.Two auxins of 2,4-dichlorophenoxyacetic acid (2,4-D)and 3,6-dichloro-o-anisic acid(dicamba)were evaluated for highly significant effect on both callus and plantlet production,although they were genotype-dependent in wheat.Substitution of 2,4-D by dicamba enhanced the growth and regeneration ability of callus from the immature embryos of most genotypes tested.The callus growth state couldn’t be modified by adding antioxidants such as ascorbic acid,cysteine,and silver nitrate or organic additives such as thiamine HCl and asparagine to the media.On the contrary,the best tissue statement and plant regeneration was achieved by employing the media containing the simplest MS(Murashige and Skoog)components and dicamba without organic components and vitamins.Thereby,our results are thought to inhibit wheat cell necrosis effectively and suggested to be used for more wheat genotypes.
学科主题	植物保护
语种	英语
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/106132]
专题	作物科学研究所
作者单位	Institute of Crop Sciences,Chinese Academy of Agricultural Sciences, Key Laboratory of Crop Genetics and Breeding,Ministry of Agriculture, Beijing, 100081
推荐引用方式 GB/T 7714	TAO Li-li,YIN Gui-xiang,DU Li-pu,et al. Improvement of Plant Regeneration from Immature Embryos of Wheat Infected by Agrobacterium tumefaciens[J]. Agricultural Sciences in China,2011,10(3):317-326.
APA	TAO Li-li.,YIN Gui-xiang.,DU Li-pu.,SHI Zheng-yuan.,SHE Mao-yun.,...&YE Xing-guo.(2011).Improvement of Plant Regeneration from Immature Embryos of Wheat Infected by Agrobacterium tumefaciens.Agricultural Sciences in China,10(3),317-326.
MLA	TAO Li-li,et al."Improvement of Plant Regeneration from Immature Embryos of Wheat Infected by Agrobacterium tumefaciens".Agricultural Sciences in China 10.3(2011):317-326.